Production of syringotoxin and other bioactive peptides byPseudomonas fuscovaginae
Abstract The application of a cell-free extract from a broth culture of Pseudomonas fuscovaginae to rice plants produced leaf sheath necrosis and inhibited panicle emergence typical of bacterial sheath rot. The extract was also fungistatic to Geotrichum candidum and displayed amphipathic properties. The toxic activity was associated with peptidic substances of less than 5000Da, migrating in a single broad band on SDS–PAGE. The toxins were precipitated by desoxycholate/trichloroacetic acid and reverse-phase HPLC of the precipitate showed the presence of five peptidic compounds (A, B, C, D and E), as well as two others (fuscopeptins A and B) which were not characterized in this study. Using FAB-MS, the MH + ions of the peptides, A to E were 1108, 1102, 1118, 1136 and 1152, respectively. The most abundant peptide, D, was characterized by amino acid analysis, fatty acid methyl ester analysis and FAB-MS of the by-products from mild alkaline hydrolysis. The chemical structure of D was identical to syringotoxin (C 48 H 82 O 18 N 11 Cl), a phytotoxin produced by P. syringae pv. syringae. Moreover, HPLC analysis of this compound indicated that compound D had the same retention time as the syringotoxin standard obtained from citrus strains of P. syringae pv. syringae.
원문보기 무료다운로드 유료다운로드
- 원문이 없습니다.
유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.
NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.