How accurate is the determination of blood in gastric juice? Comparison of peroxidase and porphyrin methods.
BACKGROUND: Mucosal bleeding is frequently used as a measure of gastric mucosal integrity. We compared the orthotolidine method for quantifying haemoglobin in gastric juice with a method based on measurement of fluorescent porphyrins extracted from haem. We also investigated whether acid or pepsin had a deleterious effect on the results of either method. METHODS: We compared the effects of pH (2 or 7), time (up to 180 min), haemoglobin concentration and the addition of pepsin (2.5, 5 or 7.5 kU/mL hog pepsin) on the accuracy of the two methods. RESULTS: With the orthotolidine method there was a time-dependent decline in detectable haemoglobin concentration (P
원문보기 무료다운로드 유료다운로드
- 무료 원문 정보가 존재하지 않습니다.
- DOI : http://dx.doi.org/10.1111/j.0953-0673.1996.00333.x
- EBSCO Industries, Inc. : 저널
- Blackwell Publishing_STM : 저널 > 논문
NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.