Preparation and characterization of a twin-tailed, lipid-linked polysialic acid
Reactions between water-insoluble and water-soluble components are often slow, giving low yields. In order to prepare glycolipids composed of polysialic acid and N-(4-(p-maleimidylphenyl) butyryl)dipalmitoyl-L-α- phosphatidylethanol amine triethylammonium salt, we used liposomes to enhance the interfacial reaction between the reagents. Thus, thiol-reactive liposomes were reacted with polysialic acid cysteamine. A 70% yield was achieved with this addition reaction, and the product was isolated by dialysis and centrifugation.(Author abstract)
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