Complex formation of tunichrome Mm-2 with ferrous and ferric ions
Abstract Mm-2, a tunichrome from iron-accumulating tunicate, Molgular manhattensis , was synthesized and measured by uv/visible spectrophotometer for the complex formation with iron. Several portions (0.1–0.5 equivalents) of freshly prepared degassed aqueous ferrous chloride solution were added to Mn-2 in 10 mM degassed sodium phosphate buffer, pH 7.0. Absorption maximum at 325 nm of Mm-2 was disappeared upon addition of ferrous chloride with concomitant appearance of absorption peaks at 365 nm and about 590 nm. Stoichiometry of Mm-2/iron complex was estimated as about 2.5∶1. The uv/visible spectrum of free Mm-2 in which hydroxyl groups were protected with t -butyldimethylsilyl (TBDMS) groups was not changed upon addition of ferrous chloride. This result suggests that catechol moieties of tunichrome Mm-2 play an important role for the complexation reaction. The addition of ferric chloride showed an essentially identical final absorption spectrum as in ferrous chloride case except that ferric chloride should be added in excess. These preliminary results support that tunichrome Mm-2 has an ability to chelate iron and to reduce Fe(III) to Fe(II).
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- DOI : http://dx.doi.org/10.1007/BF02974068
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