A PCR primer-specific to Cylindrocarpon heteronema for detection of the pathogen in apple wood
An oligonucleotide primer (ChInt) was synthesised from the variable internally transcribed spacer (ITS) 1 region of ribosomal DNA (rDNA) of Cylindrocarpon heteronema. PCR with primers ChInt and ITS4 (from a conserved sequence of the rDNA) amplified a 470-bp fragment from several isolates of C. heteronema but not from various apple wood saprophytes. Amplification of this fragment was achieved from 1-2 pg of fungal DNA. These primers amplified a fragment of the same size from DNA extracted from cankered wood but only after impurities were removed from the DNA on a Qiagen tip-5 column. Southern hybridization analysis confirmed the 470-bp fragment from C. heteronema DNA and cankered wood to be identical.
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- DOI : http://dx.doi.org/10.1016/0378-1097(93)90497-P
- Oxford University Press : 저널 > http://femsle.oxfordjournals.org/content/108/1/117.abstract
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