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Journal of Korean medical science : JKMS v.19 no.6 = no.100, 2004년, pp.834 - 841  
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In vivo Recombinant Adenovirus-mediated p53 Gene Therapy in a Syngeneic Rat Model for Colorectal Cancer

Baek, Jeong-Heum    (Department of Surgery, Gachon Medical School, Gil Medical Center, Incheon, Korea.   ); Agarwal, Munna L    (Department of Molecular Biology, The Cleveland Clinic Foundation, USA.   ); Tubbs, Raymond R    (Department of Clinical Pathology, The Cleveland Clinic Foundation, USA.   ); Vladisavljevic, Alex    (Department of Surgery, New York Presbyterian Hospital Weill Cornell Medical Center, USA.   ); Tomita, Hiroshi    (Department of Colorectal Surgery, The Cleveland Clinic Foundation, USA.   ); Bukowski, Ronald M    (Department of Hematology & Medical Oncology, The Cleveland Clinic Foundation, USA.   ); Milsom, Jeffrey W.    (Department of Surgery, New York Presbyterian Hospital Weill Cornell Medical Center, USA.   ); Kim, Jin-Man    (Department of Pathology, Chungnam National University, Daejon, Korea.   ); Kwak, Jin-Young    (Department of Surgery, Hanyang University, Seoul, Korea.  );
  • 초록

    The p53 gene has a significant role in controlling genomic stability of cancer. The purpose of this study was to evaluate the tumor response of allograft colorectal tumor treated with Ad5CMV-p53 in a syngeneic rat model. Two weeks after the inoculation of WB-2054-M5 tumor cells in the flank of rats, rats were randomly assigned by tumor size to one of three groups (n=18 in each): phosphate buffered saline (PBS), Ad5CMV, and Ad5CMV-p53. Recombinant adenovirus or PBS was administered through intratumoral injection at three divided doses every other day for 4 weeks. Apoptosis of the tumors was evaluated using TUNEL assay. After 2 and 4 weeks of treatment, the volume (cm 3 ) of tumors in PBS, Ad5CMV, and Ad5CMV-p53 was as follows: 2 week: 1.66±0.43, 1.40±0.47, 0.75±0.26 ( p p p = 0.32). The TUNEL assay results revealed more apparent apoptotic cells in Ad5CMV-p53-treated tumors than in other groups. Growth of allograft colorectal cancer in the syngeneic rat model was significantly suppressed by intratumoral Ad5CMV-p53 gene therapy. These results demonstrate that gene replacement therapy with p53 may provide a novel modality of treatment in conjunction with other present treatments for metastatic colorectal cancer.


  • 주제어

    Colorectal Neoplasms .   Gene Therapy .   Protein p53 .   Recombinant Adenovirus .   Transgenes.  

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