본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Comparison of Asthma Phenotypes Using Different Sensitizing Protocols in Mice

Chang, Yoon-Seok    (Department of Internal Medicine, College of Medicine, Seoul National University Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, and Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.   ); Kim, Yoon-Keun    (Department of Internal Medicine, College of Medicine, Seoul National University Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, and Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.   ); Bahn, Joon-Woo    (Department of Internal Medicine, College of Medicine, Seoul National University Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, and Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.   ); Kim, Sang-Heon    (Department of Internal Medicine, College of Medicine, Seoul National University Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, and Clinical Research Institute, Seou  ); Park, Heung-Woo   Kim, Tae-Bum   Cho, Sang-Heon   Min, Kyung-Up   Kim, You-Young  
  • 초록

    Background Several methods have been reported to induce asthmatic reactions in mice but few studies have compared their efficiency. We evaluated the efficiency of the protocols frequently used in the literature. Methods BALB/c mice were sensitized to ovalbumin (OVA) by intraperitoneal injection; 1] Once a week for two weeks using OVA with alum (IPOA-2) or without (IPO-2), and provocation on days 28-30 by 1% OVA inhalation; 2] seven times for two weeks by OVA with alum (IPOA-7) or without (IPO-7) and provocation by 1% OVA inhalation on days 42-44. 3] Sensitization by 1% OVA inhalation for ten days (IHO-10) and provocation by 1% OVA inhalation on days 28-30. After the last challenge, airway hyperresponsiveness was measured with single chamber plethysmography 24 hours later and mice were sacrificed 48 hours later. Results Airway hyperresponsiveness, BALF eosinophilia, airway inflammation, and OVA-specific IgE and IgG1 production were effectively induced in IPOA-2, IPOA-7, and IPO-7. However, these phenotypes were not induced in IPO-2 (except for increased BALF eosinophils) or IHO-10 (except for an increased OVA-specific IgG1 level). Conclusion The intraperitoneal injections of OVA with alum once a week for two weeks proved to be the most efficient sensitization method of inducing an asthmatic reaction in mice.


  • 주제어

    Allergy .   Asthma .   Disease models .   Animal .   Mice .   Efficiency.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기