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Journal of Korean medical science : JKMS v.21 no.6 = no.112, 2006년, pp.1000 - 1004  
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Cotransplanted Bone Marrow Derived Mesenchymal Stem Cells (MSC) Enhanced Engraftment of Hematopoietic Stem Cells in a MSC-dose Dependent Manner in NOD/SCID Mice

Kim, Dong Hyun    (School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.   ); Yoo, Keon Hee    (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.   ); Yim, Young Sook    (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.   ); Choi, Jaewon    (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.   ); Lee, Soo Hyun    (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.   ); Jung, Hye Lim    (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.   ); Sung, Ki Woong    (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.   ); Yang, Sung-Eun    (Department of Research and Development for Cellular Therapy, Medipost Biomedical Research Institute, Seoul, Korea.   ); Oh, Won Il    (Department of Research and Development for Cellular Therapy, Medipost Biomedical Research Institute, Seoul, Korea.   ); Yang, Yoon-Sun    (Department of Research and De  ); Kim, Sang-Hee   Choi, Sang-Yun   Koo, Hong Hoe  
  • 초록

    Transplantation of marrow-derived mesenchymal stem cells (MSCs), expanded by culture in addition to whole bone marrow, has been shown to enhance engraftment of human hematopoietic stem cells (HSCs). Our hypothesis was that there might be an optimum ratio range that could enhance engraftment. We examined the percent donor chimerism according to the ratio of HSCs to MSCs in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. We tested a series of ratios of co-transplanted CD34+-selected bone marrow cells, and marrow-derived MSCs into sublethally irradiated NOD/SCID mice. In all experiments, 1×10 5 bone marrow derived human CD34+ cells were administered to each mouse and human MSCs from different donors were infused concomitantly. We repeated the procedure three times and evaluated engraftment with flow cytometry four weeks after each transplantation. Serial ratios of HSCs to MSCs were 1:0, 1:1, 1:2 and 1:4, in the first experiment, 1:0, 1:1, 1:2, 1:4 and 1:8 in the second and 1:0, 1:1, 1:4, 1:8 and 1:16 in the third. Cotransplantation of HSCs and MSCs enhanced engraftment as the dose of MSCs increased. Our results suggest that the optimal ratio of HSCs and MSCs for cotransplantation might be in the range of 1:8-1:16; whereas, an excessive dose of MSCs might decrease engraftment efficiency.


  • 주제어

    Hematopoietic Stem Cells .   Mesenchymal Stem Cells .   Transplantation .   Mice, SCID .   Engraftment.  

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