본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of veterinary science v.8 no.3, 2007년, pp.255 - 261  

Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro

Jung, Tae S.    (Laboratory of Fish and Shellfish Diseases, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea.   ); Thompson, Kim D.    (Aquatic Vaccine Unit, Institute of Aquaculture, University of Stirling, Stirling, FK9 4LA, Scotland, UK.   ); Volpatti, Donatella    (Dipartimento di Scienze della Produzione Animale, Faculta Di Medicina Veterinaria, Universita degli studi di Udine, 20B 33100 Udine, Italy.   ); Galeotti, Marco    (Dipartimento di Scienze della Produzione Animale, Faculta Di Medicina Veterinaria, Universita degli studi di Udine, 20B 33100 Udine, Italy.   ); Adams, A.    (Aquatic Vaccine Unit, Institute of Aquaculture, University of Stirling, Stirling, FK9 4LA, Scotland, UK.  );
  • 초록

    The antigenicity of Photobacterium damselae ( Ph. d. ) subsp. piscicida , cultured in four different growth media [tryptone soya broth (TSB), glucose-rich medium (GRM), iron-depleted TSB (TSB + IR - ), and iron-depleted GRM (GRM + IR - )] was compared by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis using sera obtained from sea bass ( Dicentrarchus labrax ) raised against live or heat-killed Ph. d. subsp. piscicida . The antigenic expression of Ph. d. subsp. piscicida was found to differ depending on the culture medium used. A significantly higher antibody response was obtained with iron-depleted bacteria by ELISA compared with non-iron depleted bacteria obtained from the sera of sea bass raised against live Ph. d. subsp. piscicida . The sera from sea bass raised against live bacteria showed a band at 22 kDa in bacteria cultured in TSB + IR - or GRM+ IR - when bacteria that had been freshly isolated from fish were used for the screening, while bands at 24 and 47 kDa were observed with bacteria cultured in TSB or GRM. When bacteria were passaged several times on tryptic soya agar prior to culturing in the four different media, only bands at 24 and 47 kDa were recognized, regardless of the medium used to culture the bacteria. It would appear that the molecular weight of Ph. d. subsp. piscicida antigens change in the presence of iron restriction, and sera from sea bass infected with live bacteria are able to detect epitopes on the antigens after this shift in molecular weight.


  • 주제어

    Dicentrarchus labrax .   glucose-rich medium .   iron depletion .   Photobacterium damselae subsp.   piscicida .   tryptone soya broth.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기