본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

ACS chemical biology v.12 no.1, 2017년, pp.124 - 131   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Genome Mining of Amino Group Carrier Protein-Mediated Machinery: Discovery and Biosynthetic Characterization of a Natural Product with Unique Hydrazone Unit

Matsuda, Kenichi (Biotechnology Research Center, The University of Tokyo, Tokyo 133-8657, ) ; Hasebe, Fumihito (Biotechnology Research Center, The University of Tokyo, Tokyo 133-8657, ) ; Shiwa, Yuh (Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, Tokyo 156-8502, ) ; Kanesaki, Yu (Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, Tokyo 156-8502, ) ; Tomita, Takeo (Biotechnology Research Center, The University of Tokyo, Tokyo 133-8657, ) ; Yoshikawa, Hirofumi (Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, Tokyo 156-8502, ) ; Shin-ya, Kazuo (National Institute of Advanced Industrial Science and Technology (AIST), Tokyo 135-0064 ) ; Kuzuyama, Tomohisa (Biotechnology Research Center, The University of Tokyo, Tokyo 133-8657, ) ; Nishiyama, Makoto (Biotechnology Research Center, The University of Tokyo, Tokyo 133-8657, ) ;
  • 초록  

    We recently revealed that a Streptomyces strain possesses the gene encoding amino group carrier protein (AmCP). AmCP is involved in the biosynthesis of a previously unidentified nonproteinogenic amino acid, (2 S ,6 R )-diamino-(5 R ,7)-dihydroxy-heptanoic acid (DADH), which is a core compound for the synthesis of the dipeptide-containing novel natural product vazabitide A. We used polymerase chain reaction (PCR) screening to investigate the diversity of the biosynthetic machinery that uses AmCP; the results revealed that genes encoding AmCP are widely distributed among actinomycetes. The heterologous expression of the AmCP-containing gene cluster from Streptomyces sp. SoC090715LN-17 led to the discovery of s56-p1, a novel natural product. The structure of s56-p1 was determined by spectroscopic analysis; the results revealed that s56-p1 has a putative DADH-derived molecule as the core and also possesses a unique hydrazone unit that is rarely observed in natural products. Our results pave the way for investigations of unexploited AmCP-mediated biosynthesis routes among actinomycetes and of the biosynthetic mechanism of the unique hydrazone unit. Graphic Abstract ACS Electronic Supporting Info


 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기