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ACS applied materials & interfaces v.9 no.2, 2017년, pp.1352 - 1360   SCI SCIE
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Multicopper Laccase Mimicking Nanozymes with Nucleotides as Ligands

Liang, Hao (State key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, ); Lin, Feifei ( State key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, ); Zhang, Zijie ( Department of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, ); Liu, Biwu ( Department of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, ); Jiang, Shuhui ( State key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, ); Yuan, Qipeng ( State key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, ); Liu, Juewen ( Department of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, );
  • 초록  

    Using nanomaterials to achieve functional enzyme mimics (nanozymes) is attractive for both applied and fundamental research. Laccases are multicopper oxidases highly important for biotechnology and environmental remediation. In this work, we report an exceptionally simple yet functional laccase mimic based on guanosine monophosphate (GMP) coordinated copper. It forms an amorphous metal–organic framework (MOF) material. The ratio of copper and GMP is 3:4 as determined by isothermal titration calorimetry. It has excellent laccase-like activity and converts a diverse range of phenol containing substrates such as hydroquinone, naphthol, catechol and epinephrine. Comparative work shows that the activity is originated from guanosine coordination instead of phosphate binding in GMP. Cu 2+ is required and cannot be substituted by other metal ions. At the same mass concentration, the Cu/GMP nanozyme has a higher V max and similar K m compared to the protein laccase. To achieve the same catalytic efficiency, the cost of the Gu/GMP is ∼2400-fold lower than that of laccase. The Cu/GMP is much more stable at extreme pH, high salt, high temperature and for long-term storage. This is one of the first laccase-mimicking nanozymes, which will find important applications in analytical chemistry, environmental protection, and biotechnology. Graphic Abstract ACS Electronic Supporting Info


  • 주제어

    laccase .   metal−organic frameworks .   nucleotides .   copper .   nanozymes.  

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