본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Analytical chemistry v.89 no.2, 2017년, pp.1194 - 1201   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

The “PepSAVI-MS” Pipeline for Natural Product Bioactive Peptide Discovery

Kirkpatrick, Christine L. (Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, ) ; Broberg, Christopher A. (Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, ) ; McCool, Elijah N. (Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, ) ; Lee, Woo Jean (Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, ) ; Chao, Alex (Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, ) ; McConnell, Evan W. (Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, ) ; Pritchard, David A. (Department of Biostatistics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, ) ; Hebert, Michael (Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, ) ; Fleeman, Renee (Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, Florida, ) ; Adams, Jessie (Department of Cell Biology, Microbiology and Molecular Biology, University of ) ; Jamil, Amer ; Madera, Laurence ; Strömstedt, Adam A. ; Göransson, Ulf ; Liu, Yufeng ; Hoskin, David W. ; Shaw, Lindsey N. ; Hicks, Leslie M. ;
  • 초록  

    The recent increase in extensively drug-resistant bacterial pathogens and the associated increase of morbidity and mortality demonstrate the immediate need for new antibiotic backbones with novel mechanisms of action. Here, we report the development of the PepSAVI-MS pipeline for bioactive peptide discovery. This highly versatile platform employs mass spectrometry and statistics to identify bioactive peptide targets from complex biological samples. We validate the use of this platform through the successful identification of known bioactive peptides from a botanical species, Viola odorata . Using this pipeline, we have widened the known antimicrobial spectrum for V. odorata cyclotides, including antibacterial activity of cycloviolacin O2 against A.?baumannii . We further demonstrate the broad applicability of the platform through the identification of novel anticancer activities for cycloviolacins by their cytotoxicity against ovarian, breast, and prostate cancer cell lines. Graphic Abstract ACS Electronic Supporting Info


 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기