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Analytical chemistry v.89 no.2, 2017년, pp.1373 - 1381   SCI SCIE
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Virus-Enabled Biosensor for Human Serum Albumin

Ogata, Alana F. (Department of Chemistry, University of California, Irvine, Irvine, California 92697-2025, ); Edgar, Joshua M. ( Department of Chemistry, University of California, Irvine, Irvine, California 92697-2025, ); Majumdar, Sudipta ( Department of Chemistry, University of California, Irvine, Irvine, California 92697-2025, ); Briggs, Jeffrey S. ( PhageTech Inc., 5151 California Avenue, Suite 150, Irvine, California 92617, ); Patterson, Shae V. ( PhageTech Inc., 5151 California Avenue, Suite 150, Irvine, California 92617, ); Tan, Ming X. ( Wainamics Inc., 3135 Osgood Court, Fremont, California 94539, ); Kudlacek, Stephan T. ( Department of Chemistry, University of California, Irvine, Irvine, California 92697-2025, ); Schneider, Christine A. ( Department of Chemistry, University of California, Irvine, Irvine, California 92697-2025, ); Weiss, Gregory A. ( Department of Chemistry, University of California, Irvine, Irvine, California 92697-2025, ); Penner, Reginald M. ( Department of Chemistry, University of California, Irvine, Irvine, California 92697-2025, );
  • 초록  

    The label-free detection of human serum albumin (HSA) in aqueous buffer is demonstrated using a simple, monolithic, two-electrode electrochemical biosensor. In this device, both millimeter-scale electrodes are coated with a thin layer of a composite containing M13 virus particles and the electronically conductive polymer poly(3,4-ethylenedioxy thiophene) or PEDOT. These virus particles, engineered to selectively bind HSA, serve as receptors in this biosensor. The resistance component of the electrical impedance, Z re , measured between these two electrodes provides electrical transduction of HSA binding to the virus-PEDOT film. The analysis of sample volumes as small as 50 μL is made possible using a microfluidic cell. Upon exposure to HSA, virus-PEDOT films show a prompt increase in Z re within 5 s and a stable Z re signal within 15 min. HSA concentrations in the range from 100 nM to 5 μM are detectable. Sensor-to-sensor reproducibility of the HSA measurement is characterized by a coefficient-of-variance (COV) ranging from 2% to 8% across this entire concentration range. In addition, virus-PEDOT sensors successfully detected HSA in synthetic urine solutions. Graphic Abstract ACS Electronic Supporting Info


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