본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Genes & development v.31 no.1, 2017년, pp.72 - 83   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM

Ingouff, Mathieu (Epigenetic Regulations and Seed Development, UMR232, Institut de Recherche pour le Dééééveloppement (IRD), Universitééééé ); Selles, Benjamin (de Montpellier, 34394 Montpellier, France ); Michaud, Caroline (Epigenetic Regulations and Seed Development, UMR232, Institut de Recherche pour le Dééééveloppement (IRD), Universitééééé ); Vu, Thiet M. (de Montpellier, 34394 Montpellier, France ); Berger, Frédééric (Epigenetic Regulations and Seed Development, UMR232, Institut de Recherche pour le Dééééveloppement (IRD), Universitééééé ); Schorn, Andrea J. (de Montpellier, 34394 Montpellier, France ); Autran, Daphnééé (Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, 1030 Vienna, Austria ); Van Durme, Matthias (Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, 1030 Vienna, Austria ); Nowack, Moritz K. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA ); Martienssen, Robert A. (Epigenetic Regulations and Seed Developmen ); Grimanelli, Daniel ( );
  • 초록  

    In this Resource/Methodology, Ingouff et al. developed a new method to investigate DNA methylation reprogramming in plants. Using Arabidopsis , the authors developed sensors that reported CG and non-CG methylation with single-cell resolution. Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA methylation is reprogrammed genome-wide during mammalian reproduction. Reprogramming is essential for zygotic totipotency and to prevent transgenerational inheritance of epimutations. However, the extent of DNA methylation reprogramming in plants remains unclear. Here, we developed sensors reporting with single-cell resolution CG and non-CG methylation in Arabidopsis. Live imaging during reproduction revealed distinct and sex-specific dynamics for both contexts. We found that CHH methylation in the egg cell depends on DOMAINS REARRANGED METHYLASE 2 (DRM2) and RNA polymerase V (Pol V), two main actors of RNA-directed DNA methylation, but does not depend on Pol IV. Our sensors provide insight into global DNA methylation dynamics at the single-cell level with high temporal resolution and offer a powerful tool to track CG and non-CG methylation both during development and in response to environmental cues in all organisms with methylated DNA, as we illustrate in mouse embryonic stem cells.


  • 주제어

    DNA methylation .   sensors .   reprogramming .   reproduction.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기