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The Journal of antimicrobial chemotherapy v.72 no.2, 2017년, pp.393 - 401   SCI SCIE
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Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant

Li, Ruichao (Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China ) ; Xie, Miaomiao (Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China ) ; Zhang, Jinfei (Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China ) ; Yang, Zhiqiang (Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China ) ; Liu, Lizhang (Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China ) ; Liu, Xiaobo (Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China ) ; Zheng, Zhiwei ; Chan, Edward Wai-Chi ; Chen, Sheng ;
  • 초록  

    Objectives To analyse and compare mcr-1 -bearing plasmids from animal Escherichia coli isolates, and to investigate potential mechanisms underlying dissemination of mcr-1 . Methods Ninety-seven ESBL-producing E. coli strains isolated from pig farms in China were screened for the mcr-1 gene. Fifteen mcr- 1-positive strains were subjected to molecular characterization and bioinformatic analysis of the mcr-1 -bearing plasmids that they harboured. Results Three major types of mcr-1 -bearing plasmids were recovered: IncX4 (∼33 kb), IncI2 (∼60 kb) and IncHI2 (∼216–280 kb), among which the IncX4 and IncI2 plasmids were found to harbour the mcr-1 gene only, whereas multiple resistance elements including bla CTX-M , bla CMY , bla TEM , fosA , qnrS , floR and oqxAB were detected, in various combinations, alongside mcr-1 in the IncHI2 plasmids. The profiles of mcr-1 -bearing plasmids in the test strains were highly variable, with coexistence of two mcr-1 -bearing plasmids being common. However, the MIC of colistin was not affected by the number of mcr-1 -carrying plasmids harboured. Comparative analysis of the plasmids showed that they contained an mcr-1 gene cassette with varied structures ( mcr-1 - orf , IS Apl1-mcr-1 - orf and Tn 6330 ), with the IncHI2 type being the most active in acquiring foreign resistance genes. A novel transposon, Tn 6330 , with the structure IS Apl1-mcr-1 - orf -IS Apl1 was found to be the key element mediating translocation of mcr-1 into various plasmid backbones through formation of a circular intermediate. Conclusions The mcr-1 gene can be disseminated via multiple mobile elements including Tn 6330 , its circular intermediate and plasmids harbouring such elements. It is often co-transmitted with other resistance determinants through IncHI2 plasmids. The functional mechanism of Tn 6330 , a typical composite transposon harbouring mcr-1 , should be further investigated.


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