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The Journal of biological chemistry v.292 no.4, 2017년, pp.1396 - 1403   SCI SCIE
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Twisting a β-Carotene, an Adaptive Trick from Nature for Dissipating Energy during Photoprotection

Llansola-Portoles, Manuel J. (From the Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Universitášíé ) ; Sobotka, Roman (Paris-Saclay, F-91198, Gif-sur-Yvette cedex, France, ) ; Kish, Elizabeth (the Centre Algatech, Institute of Microbiology, Academy of Sciences of the Czech Republic, Tášíéřeboášíéřň, 379 81, Czech Republic, and ) ; Shukla, Mahendra Kumar (From the Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Universitášíé ) ; Pascal, Andrew A. (Paris-Saclay, F-91198, Gif-sur-Yvette cedex, France, ) ; Polášívka, Tomááš (the Centre Algatech, Institute of Microbiology, Academy of Sciences of the Czech Republic, Tášíéřeboášíéřň, 379 81, Czech Republic, and ) ; Robert, Bruno (From the Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Universitášíé ) ;
  • 초록  

    Cyanobacteria possess a family of one-helix high light-inducible proteins (Hlips) that are homologous to light-harvesting antenna of plants and algae. An Hlip protein, high light-inducible protein D (HliD) purified as a small complex with the Ycf39 protein is evaluated using resonance Raman spectroscopy. We show that the HliD binds two different β-carotenes, each present in two non-equivalent binding pockets with different conformations, having their (0,0) absorption maxima at 489 and 522 nm, respectively. Both populations of β-carotene molecules were in all- trans configuration and the absorption position of the farthest blue-shifted β-carotene was attributed entirely to the polarizability of the environment in its binding pocket. In contrast, the absorption maximum of the red-shifted β-carotene was attributed to two different factors: the polarizability of the environment in its binding pocket and, more importantly, to the conformation of its β-rings. This second β-carotene has highly twisted β-rings adopting a flat conformation, which implies that the effective conjugation length N is extended up to 10.5 modifying the energetic levels. This increase in N will also result in a lower S 1 energy state, which may provide a permanent energy dissipation channel. Analysis of the carbonyl stretching region for chlorophyll a excitations indicates that the HliD binds six chlorophyll a molecules in five non-equivalent binding sites, with at least one chlorophyll a presenting a slight distortion to its macrocycle. The binding modes and conformations of HliD-bound pigments are discussed with respect to the known structures of LHCII and CP29.


  • 주제어

    carotenoid .   chlorophyll .   cyanobacteria .   light-harvesting complex (antenna complex) .   photosynthesis.  

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