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Journal of clinical microbiology v.55 no.2, 2017년, pp.450 - 456   SCI SCIE
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Performance of Vitek 2 for Antimicrobial Susceptibility Testing of Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia with Vitek 2 (2009 FDA) and CLSI M100S 26th Edition Breakpoints

Bobenchik, April M. (Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA ); Deak, Eszter (Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA ); Hindler, Janet A. (UCLA Health System, Los Angeles, California, USA ); Charlton, Carmen L. (Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA ); Humphries, Romney M. (Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA );
  • 초록  

    The performances of Vitek 2 AST-GN69 and AST-XN06 cards were compared to Clinical and Laboratory Standards Institute (CLSI) reference broth microdilution (BMD) for 99 isolates of Pseudomonas aeruginosa , 26 Acinetobacter baumannii isolates, and 11 Stenotrophomonas maltophilia isolates. In total, 15 antimicrobials were evaluated, with 11 for P. aeruginosa , 14 for A. baumannii , and 2 for S. maltophilia . Categorical agreement (CA) was assessed using both Vitek 2 breakpoints and 2016 CLSI M100S 26th edition breakpoints. The essential agreement values for P. aeruginosa , A. baumannii , and S. maltophilia were 99.5%, 99.2%, and 100%, respectively. The CA values for P. aeruginosa , A. baumannii , and S. maltophilia were 94.1%, 92.7%, and 95.5%, respectively, by the Vitek 2 breakpoints, and 93.4%, 92.3%, and 95.5%, respectively, by the CLSI breakpoints. Overall, the Vitek 2 performance was comparable to that of BMD using both Vitek 2 breakpoints and 2016 CLSI M100S 26th edition breakpoints. Improved performance was noted for the reformulated piperacillin-tazobactam and imipenem found on the AST-GN69 card, with no very major or major errors noted when using the CLSI breakpoints.


  • 주제어

    Acinetobacter baumannii .   antimicrobial susceptibility testing .   Pseudomonas aeruginosa .   Stenotrophomonas maltophilia .   Vitek 2 .   breakpoints .   broth microdilution.  

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