본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of clinical microbiology v.55 no.2, 2017년, pp.535 - 544   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Zika Virus Testing Considerations: Lessons Learned from the First 80 Real-Time Reverse Transcription-PCR-Positive Cases Diagnosed in New York State

St. George, Kirsten (Wadsworth Center, New York State Department of Health, Albany, New York, USA ) ; Sohi, Inderbir S. (Division of Epidemiology, New York State Department of Health, Albany, New York, USA ) ; Dufort, Elizabeth M. (Division of Epidemiology, New York State Department of Health, Albany, New York, USA ) ; Dean, Amy B. (Wadsworth Center, New York State Department of Health, Albany, New York, USA ) ; White, Jennifer L. (Division of Epidemiology, New York State Department of Health, Albany, New York, USA ) ; Limberger, Ronald (Wadsworth Center, New York State Department of Health, Albany, New York, USA ) ; Sommer, Jamie N. (Division of Epidemiology, New York State Department of Health, Albany, New York, USA ) ; Ostrowski, Stephanie (Division of Epidemiology, New York State Department of Health, Albany, New York, USA ) ; Wong, Susan J. (Wadsworth Center, New York State Department of Health, Albany, New York, USA ) ; Backenson, P. Bryon (Division of Epidemiology, New York State Department of Health, Albany, New York, USA ) ; Kuhles, Daniel (Division of Epidemiology, New York State Department of Health, Albany, New York, USA ) ; Blog, Debra (Division of Epidemiology, New York State Department of Health, Albany, New ) ; Taylor, Jill ; Hutton, Brad ; Zucker, Howard A. ;
  • 초록  

    The performance and interpretation of laboratory tests for Zika virus (ZKV) continue to be evaluated. Serology is cross-reactive, laborious, and frequently difficult to interpret, and serum was initially solely recommended for molecular diagnosis. ZKV testing was initiated in January 2016 in New York State for symptomatic patients, pregnant women, their infants, and patients with Guillain-Barré syndrome who had traveled to areas with ZKV transmission. Subsequently, eligibility was expanded to pregnant women with sexual partners with similar travel histories. Serum and urine collected within 4 weeks of symptom onset or within 6 weeks of travel were tested with real-time reverse transcription-PCR (RT-PCR) assays targeting the ZKV envelope and NS2B genes. In this review of lessons learned from the first 80 positive cases in NYS, ZKV RNA was detected in urine only in 50 patients, in serum only in 19 patients, and in both samples concurrently in 11 patients, with average viral loads in urine a log higher than those in serum. Among 93 positive samples from the 80 patients, 41 were positive on both gene assays, 52 were positive on the envelope only, and none were positive on the NS2B only. Of the 80 infected patients, test results for 74 (93%) would have defined their infection status as not detected or equivocal if the requirement for positive results from two assay targets (two-target-positive requirement) in the initial federal guidance to public health laboratories was enforced, if urine was not tested, or if the extended eligibility time for molecular testing was not implemented. These changes facilitated more extensive molecular diagnosis of ZKV, reducing reliance on time-consuming and potentially inconclusive serology.


  • 주제어

    diagnostics .   emerging pathogens .   molecular methods .   specimen selection .   viral diagnosis.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기