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The journal of immunology : official journal of the American Association of Immunologists v.198 no.3, 2017년, pp.1034 - 1046   SCI SCIE
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Allergen Valency, Dose, and FcεRI Occupancy Set Thresholds for Secretory Responses to Pen a 1 and Motivate Design of Hypoallergens

Mahajan, Avanika (Department of Pathology, University of New Mexico, Albuquerque, NM 87131 ) ; Youssef, Lama A. (Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, Damascus University, Damascus, Syria ) ; Cleyrat, Cédric (Department of Pathology, University of New Mexico, Albuquerque, NM 87131 ) ; Grattan, Rachel (Department of Pathology, University of New Mexico, Albuquerque, NM 87131 ) ; Lucero, Shayna R. (Department of Pathology, University of New Mexico, Albuquerque, NM 87131 ) ; Mattison, Christopher P. (Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, New Orleans, LA 70124 ) ; Erasmus, M. Frank (Department of Pathology, University of New Mexico, Albuquerque, NM 87131 ) ; Jacobson, Bruna (Department of Computer Sciences, University of New Mexico, Albuquerque, NM 87131 ) ; Tapia, Lydia (Department of Computer Sciences, University of New Mexico, Albuquerque, NM 87131 ) ; Hlavacek, William S. (Theoretical Biology and Biophysics Group, Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM 87545 ) ; Schuyler, Mark (Department of Medicine, University of New Mexico, Albuquerque, NM 87131 ) ; Wilson, Bridget S. (Department of Pa ) ;
  • 초록  

    Ag-mediated crosslinking of IgE–FcεRI complexes activates mast cells and basophils, initiating the allergic response. Of 34 donors recruited having self-reported shrimp allergy, only 35% had significant levels of shrimp-specific IgE in serum and measurable basophil secretory responses to rPen a 1 (shrimp tropomyosin). We report that degranulation is linked to the number of FcεRI occupied with allergen-specific IgE, as well as the dose and valency of Pen a 1. Using clustered regularly interspaced palindromic repeat–based gene editing, human RBL rαKO cells were created that exclusively express the human FcεRIα subunit. Pen a 1–specific IgE was affinity purified from shrimp-positive plasma. Cells primed with a range of Pen a 1–specific IgE and challenged with Pen a 1 showed a bell-shaped dose response for secretion, with optimal Pen a 1 doses of 0.1–10 ng/ml. Mathematical modeling provided estimates of receptor aggregation kinetics based on FcεRI occupancy with IgE and allergen dose. Maximal degranulation was elicited when ∼2700 IgE–FcεRI complexes were occupied with specific IgE and challenged with Pen a 1 (IgE epitope valency of ≥8), although measurable responses were achieved when only a few hundred FcεRI were occupied. Prolonged periods of pepsin-mediated Pen a 1 proteolysis, which simulates gastric digestion, were required to diminish secretory responses. Recombinant fragments (60–79 aa), which together span the entire length of tropomyosin, were weak secretagogues. These fragments have reduced dimerization capacity, compete with intact Pen a 1 for binding to IgE–FcεRI complexes, and represent a starting point for the design of promising hypoallergens for immunotherapy.


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