본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Proceedings of the National Academy of Sciences of the United States of America v.114 no.4, 2017년, pp.699 - 704   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Steroid signaling in mature follicles is important for Drosophila ovulation

Knapp, Elizabeth (Department of Physiology & Neurobiology, University of Connecticut, Storrs, CT 06269 ); Sun, Jianjun (Department of Physiology & Neurobiology, University of Connecticut, Storrs, CT 06269; );
  • 초록  

    Significance Ovulation is thought to be divergent from insects to mammals. In the latter case, ovulation is regulated by luteinizing hormone and the steroid hormone progesterone, neither of which is found in insects so far. This paper reports a role of ecdysteroids in Drosophila ovulation. Similar to the action of progesterone in mammals, 20-hydroxyecdysone produced in Drosophila follicle cells activates one of its receptors, EcR.B2, to allow proper activation of matrix metalloproteinase and follicle rupture. The conservation of steroid signaling in ovulation makes Drosophila a simple model to define the mechanism of steroid action in ovulation. Although ecdysteroid signaling regulates multiple steps in oogenesis, it is not known whether it regulates Drosophila ovulation, a process involving a matrix metalloproteinase-dependent follicle rupture. In this study, we demonstrated that ecdysteroid signaling is operating in mature follicle cells to control ovulation. Moreover, knocking down shade ( shd ), encoding the monooxygenase that converts ecdysone (E) to the more active 20-hydroxyecdysone (20E), specifically in mature follicle cells, blocked follicle rupture, which was rescued by ectopic expression of shd or exogenous 20E. In addition, disruption of the Ecdysone receptor ( EcR ) in mature follicle cells mimicked shd -knockdown defects, which were reversed by ectopic expression of EcR.B2 but not by EcR.A or EcR.B1 isoforms. Furthermore, we showed that ecdysteroid signaling is essential for the proper activation of matrix metalloproteinase 2 (Mmp2) for follicle rupture. Our data strongly suggest that 20E produced in follicle cells before ovulation activates EcR.B2 to prime mature follicles to be responsive to neuronal ovulatory stimuli, thus providing mechanistic insights into steroid signaling in Drosophila ovulation.


  • 주제어

    steroid signaling .   ecdysone .   ovulation .   Shade .   EcR isoforms.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기