Metabolic profiling reveals a coordinated response of isolated lamb's (Valerianella locusta, L.) lettuce cells to sugar starvation and low oxygen stress
Abstract Sugar starvation is a common phenomenon occurring in most leafy vegetables after harvest and storage. Additionally, leafy vegetables are subjected to low O 2 stress when stored in modified atmosphere conditions. In this study, the metabolism of isolated lamb’s lettuce cells was studied upon sugar starvation under O 2 stress conditions, using 13 C labelled glucose. Fast depletions of the soluble sugars were observed, being more pronounced under aerobic conditions than under low O 2 stress conditions. Sugar starvation under aerobic conditions resulted in increased levels and decreased 13 C label incorporation of TCA cycle intermediates and amino and fatty acids originating from glycolytic and TCA cycle pathways, compared to starving cells incubated under low O 2 stress. On incubation under low O 2 stress a switch in metabolism from aerobic to fermentation metabolism was observed. Under low O 2 stress conditions, increased levels and 13 C label incorporated in hexose phosphates, pyruvate, lactate, GABA, alanine, together with increased levels of acetaldehyde, ethanol and ethyl acetate was observed indicating fermentative metabolism was triggered. Highlights Leafy vegetables are stored in MAP and CA to preserve quality. Leafy vegetables are subjected to sugar starvation in addition to low oxygen stress. Sugar starvation is slowed down under low oxygen stress. Under low oxygen stress, no switch to alternative sources of substrate. Switch from aerobic to fermentation metabolism.
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- DOI : http://dx.doi.org/10.1016/j.postharvbio.2016.12.004
- Elsevier : 저널> 권호 > 논문
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