Diagnosis of Mediterranean visceral leishmaniasis by detection of Leishmania-related antigen in urine and oral fluid samples
Abstract Implementation of simple diagnostic tests using non-invasive collection of biological specimens is of great importance in the diagnosis of pediatric visceral leishmaniasis caused by Leishmania infantum . Latex agglutination kit (KAtex ? ) is widely used in the diagnosis mainly in L. donovani endemic areas. However its utilization in L. infantum endemic regions remains limited and its use on noninvasive biological specimen apart urine was not reported. In this study, KAtex ? kit was used to detect Leishmania -related antigen in urine and oral fluid of 35 L. infantum visceral leishmaniasis cases and 62 controls including non-infectious disease and infectious disease controls (34 and 28 respectively). Sensitivity and specificity of urine based KAtex ? were 51.4% and 98.3% respectively, whereas, sensitivity and specificity of oral-fluid based KAtex ? were 80% and 88.3% respectively. Although, sensitivity of oral-fluid KAtex ? was high, its specificity varied significantly according to the presence or the absence of an infectious disease (71.4% versus 97%, p=0.01). Highlights Sensitivity and specificity of KAtex? kit in the diagnosis of visceral leishmaniasis were 51.4% and 98.3% respectively. Sensitivity and specificity of KAtex? kit using oral-fluid were 80% and 88.3% respectively. Lack of specificity in oral fluid is probably due to cross-reaction with soluble bacteria related-antigen. Graphical abstract Sensitivity and specificity of KAtex? kit in the diagnosis of visceral leishmaniasis caused by Leishmania infantum varied according to biological sample used (urine or oral-fluid). [DISPLAY OMISSION]
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- DOI : http://dx.doi.org/10.1016/j.actatropica.2016.12.026
- Elsevier : 저널> 권호 > 논문
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