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International journal of medical microbiology : IJMM v.307 no.1, 2017년, pp.44 - 56   SCI SCIE
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Strain-specific impact of the high-pathogenicity island on virulence in extra-intestinal pathogenic Escherichia coli

Smati, Mounira (INSERM, IAME, UMR1137, Paris, France ); Magistro, Giuseppe ( Department of Urology, Ludwig-Maximilians-University of Munich, Munich, Germany ); Adiba, Sandrine ( Institut de Biologie de l'Ecole Normale Supé ); Wieser, Andreas (rieure, ENS, CNRS UMR8197, INSERM U1024, Paris, France ); Picard, Bertrand ( Max von Pettenkofer-Institut fü ); Schubert, Sö (r Hygiene und Medizinische Mikrobiologie, Mü ); ren (nchen, Germany ); Denamur, Erick ( INSERM, IAME, UMR1137, Paris, France );
  • 초록  

    Abstract In order to clarify the role of the high-pathogenicity island (HPI) in the experimental virulence of Escherichia coli , we constructed different deletion mutants of the entire HPI and of three individual genes ( irp2, fyuA and ybtA ), encoding for three main functions within the HPI. Those mutants were constructed for three phylogroup B2 strains (536-STc127, CFT073-STc73, and NU14-STc95), representative of the main B2 subgroups causing extra-intestinal infections. Transcriptional profiles obtained for the selected HPI genes irp2 , fyuA and ybtA revealed similar patterns for all strains, both under selective iron-deplete conditions and in intracellular bacterial communities in vitro , with a high expression of irp2 . Deletion of irp2 and ybtA abrogated yersiniabactin production, whereas the fyuA knockout was only slightly impaired for siderophore synthesis. The experimental virulence of the strains was then tested in amoeba Dictyostelium discoideum and mouse septicaemia models. No effect of any HPI mutant was observed for the two more virulent strains 536 and CFT073. In contrast, the virulence of the less virulent NU14 strain was dramatically diminished by the complete deletion of the HPI and irp2 gene whereas a lesser reduction in virulence was observed for the fyuA and ybtA deletion mutants. The two experimental virulence models gave similar results. It appears that the role of the HPI in experimental virulence is depending on the genetic background of the strains despite similar inter-strain transcriptional patterns of HPI genes, as well as of the functional class of the studied gene. Altogether, these data indicate that the intrinsic extra-intestinal virulence in the E. coli species is multigenic, with epistatic interactions between the genes. Highlights The effect of the HPI depends of the genetic background of the E. coli strain. The genes of different functions within the HPI differentially affect the virulence phenotype. The mouse and amoeba experimental virulence models gave similar results.


  • 주제어

    Escherichia coli .   High pathogenicity island .   Experimental virulence .   Genetic background.  

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