Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats
Polysaccharides are substances that modify the biological response to several stressors. The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats. The monosaccharide composition had a complex mixture, suggesting the presence of arabinoglactans, mannans, and pectins. Treatment with SFP (30 and 60mg/kg, oral) for 14days significantly reduced the tumor weight and volume compared with controls. Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue. Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3. The antineoplastic effect of SFP appears to be attributable to its action on the immune system by controlling the tumor microenvironment and stimulating TNF-α production, which may trigger the necroptosis pathway.
TriZol Reagent . buffered 10% formalin . ethanol . xylol . paraffin . hematoxylin and eosin (HE) . phosphate buffer (pH 6. 5) . Griess solution (0. 1% N-1-naphthyl-tilediamine, 1% sulfanilamide in 5% H3PO4) . saline Triton X-100 0. 1%, TMB 18. 4@?mM . dimethylformamide 8% . sodium acetate (NaOAc) . p-nitrophenyl-N-acetyl-β-d-glucosamine . N-acetyl-β-d-glucosamine . p-nitrofen . ketamine hydrochloride . xylazine hydrochloride; . metothrexate . phosphate buffered saline (PBS, 16. 5@?mM phosphate, 137@?mM NaCl, and 2. 7@?mM KCl) at pH 7. 4 . ethylenediaminetetraacetic acid (EDTA) (0. 5@?M, pH 8. 0) . eTrypan blue, 2@?M trifluoroacetic acid . Walker-256 tumor . polysaccharide . red wine . cabernet franc . necroptosis . immunomodulation.
NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.
- 이 논문과 함께 출판된 논문 + 더보기