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Molecular immunology v.82, 2017년, pp.104 - 113  

Immunization with Leishmania donovani protein disulfide isomerase DNA construct induces Th1 and Th17 dependent immune response and protection against experimental visceral leishmaniasis in Balb/c mice

Amit, A. ; Vijayamahantesh ; Dikhit, M.R. ; Singh, A.K. ; Kumar, V. ; Suman, S.S. ; Singh, A. ; Kumar, A. ; Thakur, A.K. ; Das, V.R. ; Das, P. ; Bimal, S. ;
  • 초록  

    In the present study, the efficacy of Leishmania donovani protein disulfide isomerase (LdPDI) as a DNA vaccine was evaluated in BALB/C mice. Mice immunized with the LdPDI-DNA construct were found to be the most immuno-reactive, as the construct induced higher T-cell proliferation. The increased T-cell proliferation was associated with a substantial rise in Th1 and Th17+ CD4 cell response and triggered a higher proportion of CD8+ T cells for the release of interferon-gamma along with a reduced splenic parasite load on Days20 and 60 post challenge (PC). Furthermore, the vaccine construct triggered increased interferon (IFN)-γ, interleukin(IL)-17A, and IL-22 release accompanied by decreased extracellular signal-regulated kinases (ERK) ½ signaling and increased mitogen-activated protein kinase (MAPK) signaling coinciding with an increase in the amount of nitrite and reactive oxygen species (ROS)in vaccinating the splenocyts. We summarize from our data that the PDI-DNA construct of Leishmania donovani has the potential to elicit protective immunity through the pro-inflammatory cytokines of CD8+ and CD4+(Th1 and Th17) following an intervention in the downstream signaling event of ERK½ (probably through p38MAPK signaling). Therefore, the study suggests a new control against visceral leishmaniasis in the future.


  • 주제어

    Protein disulfide isomerase (PDI) .   DNA vaccine .   Interleukin (IL)-17A .   Nitric oxide .   Mitogen-activated protein kinase (MAPK) .   Visceral leishmaniasis .   Interferon gamma.  

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