The serine/threonine phosphatase DhSIT4 modulates cell cycle, salt tolerance and cell wall integrity in halo tolerant yeast Debaryomyces hansenii
Abstract The highly conserved family of P hos p hoprotein p hosphatases (PPP) regulates several major physiological processes in yeast. However, very little is known about the PPP orthologs from the yeast species inhabiting extreme environmental niches. In the present study we have identified DhSIT4 , a member of PPP6 class of serine threonine phosphatases from the halotolerant yeast Debaryomyces hansenii . Deletion of DhSIT4 in D . hansenii was not lethal but the mutant exhibited reduced growth due to its effect on the cell cycle. The knock out mutant Dhsit4Δ showed sensitivity towards Li + , Na + and cell wall damaging agents. The expression of DhSit4p rescued salt, caffeine and calcofluor white sensitivity of Dhmpk1Δ strain and thereby indicating a genetic interaction of this phosphatase with the cell wall integrity pathway in this species. Our study also demonstrated the antagonistic roles of DhSit4p and DhPpz1p in maintaining the cell cycle and ion homeostasis in D . hansenii . Highlights Phenotypic characterization of DhSIT4 knock out mutant of Debaryomyces hansenii DhSit4p plays important role in the cell cycle, salt tolerance and the cell wall integrity pathway. Evidence for genetic interaction between DhSit4p and DhPpz1p in regulating cell cycle, ion homeostasis and cell wall integrity
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