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Gene v.606, 2017년, pp.10 - 16   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Overexpression of miR-92a promotes the tumor growth of osteosarcoma by suppressing F-box and WD repeat-containing protein 7

Jiang, Xuesheng (Department of Orthopedics, Huzhou Central Hospital, Huzhou, Zhejiang 313003, China ) ; Li, Xiongfeng (Department of Orthopedics, Huzhou Central Hospital, Huzhou, Zhejiang 313003, China ) ; Wu, Fengfeng (Department of Orthopedics, Huzhou Central Hospital, Huzhou, Zhejiang 313003, China ) ; Gao, Hongliang (Department of Orthopedics, Huzhou Central Hospital, Huzhou, Zhejiang 313003, China ) ; Wang, Guorong (Department of Orthopedics, Huzhou Central Hospital, Huzhou, Zhejiang 313003, China ) ; Zheng, Hua (Department of Orthopedics, Huzhou Central Hospital, Huzhou, Zhejiang 313003, China ) ; Wang, Huajun (Department of Orthopedics, First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China ) ; Li, Jianyou (Department of Orthopedics, Huzhou Central Hospital, Huzhou, Zhejiang 313003, China ) ; Chen, Chao (Department of Orthopedics, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China ) ;
  • 초록  

    Abstract MicroRNAs (miRNAs) have been reported to be critical players in osteosarcoma (OS). Among numerous cancer-related miRNAs, the expression level of miR-92a and its potential role in OS has not been investigated. Here, We showed that overexpression of miR-92a was identified in OS specimens and cells compared to normal bone tissues. The high level of miR-92a was correlated with high T classification and advanced clinical stages of OS patients. Notably, miR-92a highly expressing OS patients showed a notably reduced survival rate. In vitro experiments showed that loss of miR-92a inhibited U2OS cell proliferation and cell-cycle progression while induced apoptosis. In turn, its restoration facilitated MG-63 cell growth and suppressed apoptosis. Experimental nude mice showed that miR-92a silencing prohibited the in vivo growth of OS cells. Furthermore, bioinformatics software predicted that F-box and WD repeat-containing protein 7 (FBXW7) was a direct target of miR-92a. We then observed the negative regulation of miR-92a on FBXW7 expression and the direct binding between them was further verified by dual-luciferase assays in OS cells. Forced expression of FBXW7 resulted in reduced proliferation, cell cycle arrest at G1 phase and increased apoptosis in miR-92a overexpressing MG-63 cells. In summary, this study demonstrates miR-92a probably functions as a driver of tumor progression by targeting FBXW7, and highlights the potential effects of miR-92a on prognosis and treatment of OS. Highlights miR-92a is obviously up-regulated in OS tissues and cells. High miR-92a expression correlates with poor clinical features and reduced survival. Up-regulation of miR-92a promotes OS cell growth and induces apoptosis. FBXW7 is a direct target of miR-92a. FBXW7 is implicated in elevated miR-92a-induced OS progression.


  • 주제어

    miR-92a .   Osteosarcoma .   FBXW7 .   Proliferation .   Cell cycle progression .   Apoptosis.  

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