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Renewable energy v.105, 2017년, pp.465 - 472   SCIE
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Process optimization for biodiesel production from waste cooking oil using multi-enzyme systems through response surface methodology

Babaki, Mohadese (Department of Pure Chemistry, Faculty of Chemistry, Shahid Beheshti University, G.C., Tehran, Iran ); Yousefi, Maryam ( Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran ); Habibi, Zohreh ( Department of Pure Chemistry, Faculty of Chemistry, Shahid Beheshti University, G.C., Tehran, Iran ); Mohammadi, Mehdi ( Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran );
  • 초록  

    Abstract Lipase from Rhizomucor miehei (RML) and lipase B from Candida antarctica (CALB) were covalently immobilized onto epoxy-functionalized silica. In this study, we developed a multi-enzyme system to produce biodiesel with waste cooking oil and methanol. To increase the biodiesel production yield, a mixture of 1,3-specific lipase (RML) and nonspecific lipase (CALB) was used. Response Surface Methodology (RSM) and a central composite rotatable design (CCRD) was used to study the effects of four factors, CALB:RML ratio, ratio of t -butanol to oil (wt.%), water adsorbent content (wt.%) and reaction time on the fatty acid methyl esters (FAME) yield. A quadratic polynomial equation was obtained for methanolysis reaction by multiple regression analysis. The optimum combinations for the reaction were CALB:RML ratio (3:1), t -butanol to oil (10?wt%), water adsorbent content (22.5?wt%) at the reaction time of 10?h. FAME yield of 91.5%, which was very close to the predicted value of 95.6%, was obtained. Verification experiment confirmed the validity of the predicted model. Highlights Lipases were immobilized by covalent binding onto epoxy-functionalized silica. The immobilized lipases were studied for production of biodiesel from waste cooking oil. A mixture of 1,3-specific lipase (RML) and nonspecific lipase (CALB) was used. The effect of water, t -butanol and blue silica gel on the FAME% was considered. Epoxy-functionalized silica resulted to high catalytic activity.


  • 주제어

    Biodiesel .   Lipase .   Immobilization .   Waste cooking oil .   Response surface methodology.  

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