본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of dairy science v.100 no.2, 2017년, pp.1507 - 1520   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Variable liver fat concentration as a proxy for body fat mobilization postpartum has minor effects on insulin-induced changes in hepatic gene expression related to energy metabolism in dairy cows

Weber, C. (Institute of Nutritional Physiology (“Oskar Kellner”), Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf 18196, Germany ) ; Schäff, C.T. (Institute of Nutritional Physiology (“Oskar Kellner”), Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf 18196, Germany ) ; Kautzsch, U. (Institute of Nutritional Physiology (“Oskar Kellner”), Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf 18196, Germany ) ; Börner, S. (Institute of Nutritional Physiology (“Oskar Kellner”), Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf 18196, Germany ) ; Erdmann, S. (Institute of Nutritional Physiology (“Oskar Kellner”), Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf 18196, Germany ) ; Bruckmaier, R.M. (Veterinary Physiology, Vetsuisse Faculty, University of Bern, 3001 Bern, Switzerland ) ; Röntgen, M. (Institute of Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf 18196, Germany ) ; Kuhla, B. (Institute of Nutritional Physiology (“Oskar Kellner”), Leibniz Institute for Farm Animal Biology (FBN), Du ) ; Hammon, H.M. ;
  • 초록  

    ABSTRACT The liver plays a central role in adaptation for energy requirements around calving, and changes in the effects of insulin on hepatic energy metabolism contribute to metabolic adaptation in dairy cows. Hepatic insulin effects may depend on body fat mobilization. The objective of this study was to investigate the effects of insulin on the hepatic gene expression of enzymes involved in energy metabolism and factors related to nutrition partitioning in cows with high and low total liver fat concentration (LFC) after calving. Holstein cows were retrospectively grouped according to their LFC after calving as a proxy for body fat mobilization. Cows were classified as low (LLFC; LFC 24.4% fat/dry matter; n = 10) fat-mobilizing after calving. Euglycemic-hyperinsulinemic clamps [6 mU/(kg × min) of insulin for 6 h] were performed in wk 5 antepartum (ap) and wk 3 postpartum (pp). Before and at the end of the euglycemic-hyperinsulinemic clamps, liver biopsies were taken to measure the mRNA abundance of enzymes involved in carbohydrate and lipid metabolism, expression related to the somatotropic axis, and adrenergic and glucocorticoid receptors. The mRNA abundance of pyruvate carboxylase, cytosolic phosphoenolpyruvate carboxykinase (PEPCK; PCK1 ), acyl-CoA-dehydrogenase very long chain ( ACADVL ), and hydroxyl-methyl-glutaryl-CoA-synthase 1 increased, but the mRNA abundance of solute carrier family 2 ( SLC2A2 and SLC2A4 ), growth hormone receptor 1A ( GHR1A ), insulin-like growth factor 1 ( IGF1 ), sterol regulatory element binding factor 1, adrenoceptor α 1A, and glucocorticoid receptor decreased from ap to pp. Insulin treatment was associated with decreased PCK1, mitochondrial PEPCK, glucose-6-phosphatase, propionyl-CoA-carboxylase α, carnitine-palmitoyl-transferase 1A, ACADVL , and insulin receptor mRNA, but increased IGF1 and SLC2A4 mRNA ap and pp and GHR1A mRNA pp. The mRNA abundance of SLC2A4 was greater, and the mRNA abundance of GHR1A and IGF1 tended to be lower in LLFC than in HLFC. Administration of insulin, albeit at a supraphysiological dose, was associated with inhibition of gene expression related to glucose production and β-oxidation, but we observed variable effects in the degree of insulin depression of individual genes. Insulin status is important for regulation of nutrient partitioning, but different LFC pp had very little influence on changes in hepatic gene expression following administration of insulin.


  • 주제어

    dairy cow .   insulin .   hepatic gene expression .   energy metabolism .   somatotropic axis.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기