Fructosylation induced structural changes in mammalian DNA examined by biophysical techniques
Abstract Glycosylation of DNA, proteins, lipids, etc. by reducing sugars, can lead to the formation of advanced glycation end products (AGEs). These products may accumulate and involve in the pathogenesis of a number of diseases, contributing to tissue injury via several mechanisms. In this study, fructosylation of calf thymus dsDNA was carried out with varying concentrations of fructose. The neo-structure of fructosylated-DNA was studied by various biophysical techniques and morphological characterization. Fructosylated-DNA showed hyperchromicity, increase in fluorescence intensity and decrease in melting temperature. The CD signal of modified-DNA shifted in the direction of higher wavelength indicative of structural changes in DNA. FTIR results indicated shift in specific band positions in fructosylated-DNA. Morphological characterization of fructosylated-DNA exhibited strand breakage and aggregation. The results suggest that the structure and conformation of DNA may be altered under high concentrations of fructose. Highlights Fructose treatment of dsDNA led to generation of DNA-AGEs. Fructosylated DNA was characterized by spectroscopic techniques. Single stranded breaks on fructosylated-DNA were demonstrated by agarose gel. Morphology of fructosylated-DNA was analyzed by Scanning Electron Microscope. Graphical abstract [DISPLAY OMISSION]
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