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Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy v.174, 2017년, pp.236 - 244   SCI SCIE
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Study on the interactional behaviour of transition metal ions with myoglobin: A detailed calorimetric, spectroscopic and light scattering analysis

Kaur, Amandeep (Corresponding author. ) ; Banipal, Parampaul K. ; Banipal, Tarlok S. ;
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    Abstract The energetics and the impact on the conformation of heme containing protein myoglobin (Mb) due to the binding of three transition metal ions (Zn 2+ , Ni 2+ , and Mn 2+ ) have been investigated using isothermal titration calorimetry (ITC), dynamic light scattering (DLS), UV–vis, and circular dichroism (CD) spectroscopy under physiological conditions. The binding affinity of the order of 10 4 M −1 has been observed for all metal ions from calorimetry as well as from absorption spectroscopy. The binding of these metal ions with Mb is a spontaneous process that exposes the hydrophobic groups away from the protein core as exhibited by the negative Gibbs free energy change (Δ G ) and positive heat capacity change (Δ C p ) values. Both light scattering and CD results demonstrates that the binding of Zn 2+ and Mn 2+ ions with Mb results in the folding whereas Ni 2+ ion results in the unfolding of the protein. No direct interactions among the transition metal ions and heme moiety of Mb has been observed from absorption study. The results of these studies reveals that Mn 2+ ion influences the biological functions of Mb to a larger extent in spite of its lowest affinity followed by Zn 2+ and Ni 2+ ions. Highlights Zn 2+ and Ni 2+ ions binding with Mb is exothermic whereas Mn 2+ ion binding is an endothermic process. Insignificant exposure/burial of the hydrophobic groups occurs in Mb due to binding of Mn 2+ and Zn 2+ ions. The binding of Zn 2+ and Mn 2+ ions decreases the size of Mb whereas Ni 2+ ion increases it. Zn 2+ and Mn 2+ ions provide conformational stability but Ni 2+ ion provides conformational destability to Mb. M 2+ ions do not interact with the heme moiety of Mb. Graphical Abstract [DISPLAY OMISSION]


  • 주제어

    Myoglobin .   Zinc ion .   Nickel ion .   Manganese ion .   Calorimetry .   Light scattering .   Spectroscopy.  

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