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Journal of electroanalytical chemistry v.785, 2017년, pp.117 - 124   SCI SCIE
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Electrochemical biosensor for silver ions based on amplification of DNA–Au bio–bar codes and silver enhancement

Zhang, Yanli (Key Laboratory of Comprehensive Utilization of Mineral Resources in Ethnic Regions, Yunnan Minzu University, Kunming 650031, PR China ) ; Li, Haiyan (Key Laboratory of Comprehensive Utilization of Mineral Resources in Ethnic Regions, Yunnan Minzu University, Kunming 650031, PR China ) ; Xie, Jinling (Key Laboratory of Comprehensive Utilization of Mineral Resources in Ethnic Regions, Yunnan Minzu University, Kunming 650031, PR China ) ; Chen, Meng (Key Laboratory of Comprehensive Utilization of Mineral Resources in Ethnic Regions, Yunnan Minzu University, Kunming 650031, PR China ) ; Zhang, Dandan (Key Laboratory of Comprehensive Utilization of Mineral Resources in Ethnic Regions, Yunnan Minzu University, Kunming 650031, PR China ) ; Pang, Pengfei (Key Laboratory of Comprehensive Utilization of Mineral Resources in Ethnic Regions, Yunnan Minzu University, Kunming 650031, PR China ) ; Wang, Hongbin (Key Laboratory of Comprehensive Utilization of Mineral Resources in Ethnic Regions, Yunnan Minzu University, Kunming 650031, PR China ) ; Wu, Zhan (State Key Laboratory of Chemo/Biosensing and Che ) ; Yang, Wenrong ;
  • 초록  

    Abstract A highly sensitive and selective electrochemical DNA biosensor for detection of Ag + was developed based on amplification of DNA–Au bio–bar codes and silver enhancement. Cytosine-rich single stranded DNA oligonucleotide (sub–DNA) was immobilized on the surface of the gold electrode via Au–S chemical interaction. In the presence of Ag + , with the interaction of cytosine–Ag + –cytosine (C–Ag + –C), cytosine-rich DNA oligonucleotide probes-labeled AuNPs have a hybridization with sub–DNA and then form a duplex-like structure, following the AuNPs-catalyzed silver enhancement. The whole detection procedure of Ag + based on the developed biosensor was evaluated by electrochemical techniques. Owing to the hybridization of sandwich assay format and distinct electrochemical signal amplification of silver enhancement, the proposed biosensors exhibited a good analytical performance for sensitive transduction of Ag + recognition with a wide detection linear range from 5pM to 50μM, and a low detection limit of 2pM. The developed biosensor was highly selective and its practical applicability in real water samples was also tested with satisfactory results. Highlights An electrochemical biosensor was developed for detection of silver ions. Combination of bio-bar code assay and silver enhancement could significantly amplify the electrochemical signal. Biosensor exhibits excellent sensitivity and selectivity due to high specificity of the C-Ag + -C and silver enhancement. The proposed biosensor might hold great promise in environmental monitoring of silver ions. Graphical abstract [DISPLAY OMISSION]


  • 주제어

    Silver ions .   Cytosine–Ag+–cytosine .   Bio–bar codes .   Silver enhancement .   Electrochemical biosensor.  

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