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Journal of electroanalytical chemistry v.785, 2017년, pp.125 - 130   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Electrochemical assay of proteolytically active prostate specific antigen based on anodic stripping voltammetry of silver enhanced gold nanoparticle labels

Parnsubsakul, Attasith (Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangmod, Bangkok 10140, Thailand ) ; Safitri, Rika Endara (Chemistry Department, Faculty of Mathematics and Natural Sciences, University of PGRI Banyuwangi, Banyuwangi, East Java, Indonesia ) ; Rijiravanich, Patsamon (Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand ) ; Surareungchai, Werasak (School of Bioresources and Technology, and Nanoscience & Nanotechnology Graduate Program, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand ) ;
  • 초록  

    Abstract This work demonstrated the determination of proteolytically active prostate specific antigen (paPSA), a potential biomarker for prostate cancer diagnosis, in human serum using a sensitive and low-cost electrochemical sensor. A specifically designed peptide probe was immobilized on the surface of a 96-well plate. The probe could be recognized by paPSA causing cleavage of the peptide, resulting in a decrease in the thiols group remaining on the probe. Gold nanoparticles (AuNPs) were attached to the peptide thiol groups by self-assembly. Hence the amount of AuNPs relates to the length of peptide probe. After cleavage and binding of AuNPs, an amplification step was performed using a silver enhancer solution. The quantity of deposited silver was then measured by differential pulse anodic stripping voltammetry (DPASV) using a disposable screen-printed carbon electrode (SPCE). The signal for paPSA detection was the linear range from 0.1 to 100ngmL −1 , with a detection limit of 27pgmL −1 . We also showed that the assay was reliable and has potential for clinical applications. Highlights A simple and high specific peptide sensor for proteolytically active prostate specific antigen (paPSA) detection. AuNPs induced Ag deposition and incorporating with ASV detection was used for signal amplification. Peptide was immobilized into 96-well plate for multiple samples detection. The peptide sensing shows a good analytical performance. The platform is promising for clinical applications.


  • 주제어

    paPSA .   Peptide .   Silver enhancement .   Anodic stripping voltammetry .   Screen-printed carbon electrode.  

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