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International journal of mineral processing v.159, 2017년, pp.1 - 6   SCI SCIE
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Preparation of antibacterial chlorhexidine/vermiculite and release study

Samlíková, Magda (Nanotechnology Centre, VŠB–Technical University of Ostrava, 17.listopadu 15/2172, 708 33 Ostrava, Poruba, Czech Republic ) ; Holešová, Sylva (Nanotechnology Centre, VŠB–Technical University of Ostrava, 17.listopadu 15/2172, 708 33 Ostrava, Poruba, Czech Republic ) ; Hundáková, Marianna (Nanotechnology Centre, VŠB–Technical University of Ostrava, 17.listopadu 15/2172, 708 33 Ostrava, Poruba, Czech Republic ) ; Pazdziora, Erich (Institute of Public Health Ostrava, Centre of Clinical Laboratories, Partyzánské ) ; Jankovič, Ľuboš (náměstí ) ; Valášková, Marta (7, 702 00 Ostrava, Czech Republic ) ;
  • 초록  

    Abstract The antibacterial chlorhexidine/vermiculite (CA/Ver) was successfully prepared through the intercalation process and the stability of CA on the vermiculite matrix and was investigated by stirring in aqueous solutions under the influence of different pH and temperature. The content of CA was determined by total organic carbon (TOC) analysis before and after stability tests. The structure of all samples was characterized by X-ray powder diffraction (XRD) and Fourier–transform infrared spectroscopy (FTIR). The antibacterial activity of prepared CA/Ver samples was evaluated by finding a minimum inhibitory concentration (MIC) against Enterococcus faecalis , Staphylococcus aureus , Escherichia coli and Pseudomonas aeruginosa . The content of chlorhexidine ranged from 209 to 231.6mg of CA in 1g of the whole sample after the intercalation process. After stability study, only a slight outflow of CA from the Ver matrix ( After 30min of exposition the MIC of organovermiculite samples before and after stability test were the same for Staphylococcus aureus and Escherichia coli with value 3.33 (%; w / v ) and the MIC decreased to 0.014 (%; w / v ) with longer time of exposition (120h). A small difference was observed at Enterococcus faecalis where MIC was 10 (%; w / v ) after 30min of exposition for the sample after stability test in neutral pH. However, after 24h of treatment the MIC value decreased to 0.014 (%; w / v ). And finally, bacterial strain Pseudomonas aeruginosa showed a great resistance against antibacterial organovermiculite samples and MIC did not decreased under 10 (%; w / v ) even after 5days of exposition. Highlights Antibacterial nanocomposite was successfully prepared. Stability of chlorhexidine diacetate in vermiculite matrix was investigated. Prepared nanocomposites showed good antibacterial effect against bacterial strains. CA/Ver has the potential to be used in long lasting antibacterial applications.


  • 주제어

    Vermiculite .   Chlorhexidine diacetate .   Antibacterial activity .   Stability test.  

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