The Information Content of Glutamine-Rich Sequences Define Protein Functional Characteristics
The presence of abnormally expanded glutamine (Q) repeats within specific proteins (e.g., huntingtin) is the well-established cause of several neurogenerative diseases, including Huntington disease and spinocerebellar ataxias. However, the impact of “expanded Q” stretches on the protein function is not well understood, mostly due to lack of knowledge about the physiological role of Q repeats and the mechanism by which these repeats achieve functional specificity. Indeed, it is intriguing that regions with such low complexity (low information content) can display exquisite functional specificity, prompting the question: where is this information stored? Applying biochemical/structural constraints and statistical analysis of protein composition, we identified Q-rich (Q R ) regions present in coiled coils of yeast transcription factors and endocytic proteins. Our analysis indicated the existence of non-Q amino acids (AAs) differentially enriched or excluded from Q R regions in one protein group versus the other. Importantly, when the non-Q AAs from an endocytic protein were exchanged by the ones enriched in Q R from transcription factors, the resulting protein was unable to localize to the plasma membrane and was instead found in the nucleus. These results indicate that while Q R repeats can efficiently engage in binding, the non-Q AAs provide essential specificity information. We speculate that coupling low complexity regions with information-intensive determinants might be a strategy used in many protein systems involved in different biological processes.
유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.
원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.
NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.
- 이 논문과 함께 출판된 논문 + 더보기