본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Simultaneous quantification of twenty-one ginsenosides and their three aglycones in rat plasma by a developed UFLC-MS/MS assay: Application to a pharmacokinetic study of red ginseng

Zhou, Q.L. ; Zhu, D.N. ; Yang, Y.F. ; Xu, W. ; Yang, X.W. ;
  • 초록  

    To track the pharmacokinetic features of red ginseng (RG), a rapid and sensitive ultra fast liquid chromatographic coupled with electrospray ionization triple quadrupole tandem mass spectrometry (UFLC-MS/MS) method was developed for simultaneous quantification of twenty-one ginsenosides and their three aglycones, including 18 prototype compounds (ginsenosides Rb 1 , Rb 2 , Rc, Rd, Re, Rg 1 , Rg 5 , Rh 4 , Rk 1 , Rk 3 , 20(S)-Rf, 20(S)-Rg 2 , 20?-Rg 2 , 20(S)-Rg 3 , 20?-Rg 3 , 20(S)-Rh 1 , 20?-Rh 1 , 20(S)-NG-R 2 ), and 6 metabolites (ginsenosides 20(S)-Rh 2 and Rh 3 , 20(S)-protopanaxadiol (PPD), 20(S)-protopanaxatriol (PPT), 20?-PPT, ginseng saponin compound K) of RG in rat plasma after oral administration of RG water extract at a single dose of 4g/kg body weight to rats. All analytes with internal standard (digoxin) were detected by multiple reaction monitoring in negative ionization mode and separated on an ACQUITY UPLC ? BEH RP-C 18 column (1.7μm, 100x2.1mm). This established method was well validated in terms of linearity, sensitivity, intra- and inter-day precisions, accuracy, recovery, matrix effect, stability, and had a lower limit of quantification at the concentration range of 0.12-8.12ng/mL for all of analytes. This UFLC-MS/MS approach was successfully applied to the pharmacokinetic study for RG water extract in rats. We firstly proposed that Rb 1 , Rb 2 , Rc, Rd, Rg 1 , Rg 5 , 20(S)-Rg 3 , 20(S)-Rh 2 , and 20(S)-PPD measured in rat plasma were suitable pharmacokinetic markers of RG extract in rats due to their high systemic exposure levels. Thus, this specific and reliable method will be useful for future applications to pharmacokinetic studies for various sources of ginsenoside samples and Panax herbs in vivo.


  • 주제어

    Red ginseng .   Ginsenoside .   UFLC-MS/MS .   Pharmacokinetics .   Rat plasma.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기