본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Molecular cell v.65 no.4, 2017년, pp.604 - 617.e6   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Systematic Investigation of Transcription Factor Activity in the Context of Chromatin Using Massively Parallel Binding and Expression Assays

Levo, Michal (Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel ) ; Avnit-Sagi, Tali (Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel ) ; Lotan-Pompan, Maya (Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel ) ; Kalma, Yael (Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel ) ; Weinberger, Adina (Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel ) ; Yakhini, Zohar (Computer Science Department, Technion, Haifa 3200003, Israel ) ; Segal, Eran (Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel ) ;
  • 초록  

    Summary Precise gene expression patterns are established by transcription factor (TFs) binding to regulatory sequences. While these events occur in the context of chromatin, our understanding of how TF-nucleosome interplay affects gene expression is highly limited. Here, we present an assay for high-resolution measurements of both DNA occupancy and gene expression on large-scale libraries of systematically designed regulatory sequences. Our assay reveals occupancy patterns at the single-cell level. It provides an accurate quantification of the fraction of the population bound by a nucleosome and captures distinct, even adjacent, TF binding events. By applying this assay to over 1,500 promoter variants in yeast, we reveal pronounced differences in the dependency of TF activity on chromatin and classify TFs by their differential capacity to alter chromatin and promote expression. We further demonstrate how different regulatory sequences give rise to nucleosome-mediated TF collaborations that quantitatively account for the resulting expression. Highlights Parallel expression and occupancy measurements reveal TF-nucleosome interplay Pronounced differences in transcription factors (TFs) sensitivity to chromatin Specific combinations of TF sites give rise to nucleosome-mediated collaborations Properties of nucleosome-based collaborations can quantitatively shape expression Graphical Abstract [DISPLAY OMISSION]


  • 주제어

    transcription regulation .   chromatin .   transcription factor .   promoter .   nucleosome .   gene expression .   quantitative biology .   DNA binding .   single-cell measurements .   high-throughput sequencing.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기