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Ticks and tick-borne diseases v.8 no.3, 2017년, pp.391 - 395   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation

Mihaljica, Darko (University of Belgrade, Institute for Medical Research, Centre of Excellence for Food and Vector-Borne Zoonoses, Laboratory for Medical Entomology, Dr Subotića 4, PO Box 102, 11129 Belgrade, Serbia ) ; Marković, Dragana (University of Belgrade, Institute for Medical Research, Department of Immunology, Dr Subotića 4, PO Box 102, 11129 Belgrade, Serbia ) ; Radulović, Željko (University of Belgrade, Institute for Medical Research, Centre of Excellence for Food and Vector-Borne Zoonoses, Laboratory for Medical Entomology, Dr Subotića 4, PO Box 102, 11129 Belgrade, Serbia ) ; Mulenga, Albert (Texas A&M University, College of Veterinary Medicine, Department of Veterinary Pathobiology, USA ) ; Ćakić, Sanja (University of Belgrade, Institute for Medical Research, Centre of Excellence for Food and Vector-Borne Zoonoses, Laboratory for Medical Entomology, Dr Subotića 4, PO Box 102, 11129 Belgrade, Serbia ) ; Sukara, Ratko (University of Belgrade, Institute for Medical Research, Centre of Excellence for Food and Vector-Borne Zoonoses, Laboratory for Medical Entomol ) ; Samardžić, Jelena ; Tomanović, Snežana ;
  • 초록  

    Abstract Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus ( Ir ) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that r Ir AV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.


  • 주제어

    Tick bite markers .   Salivary proteins .   Exposure to ticks .   Ixodes ricinus.  

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