본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Ticks and tick-borne diseases v.8 no.3, 2017년, pp.400 - 406   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Molecular characterisation of Anaplasma species from African buffalo (Syncerus caffer) in Kruger National Park, South Africa

Sisson, Danielle (Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria, Australia ) ; Hufschmid, Jasmin (Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria, Australia ) ; Jolles, Anna (College of Veterinary Medicine, Oregon State University, Corvallis, USA ) ; Beechler, Brianna (College of Veterinary Medicine, Oregon State University, Corvallis, USA ) ; Jabbar, Abdul (Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria, Australia ) ;
  • 초록  

    Abstract Bovine anaplasmosis is a tick-borne disease, mainly caused by Anaplasma marginale and A. centrale and is distributed in tropical and sub-tropical areas. This study aimed to characterise A. marginale and A. centrale from African buffaloes in Kruger National Park (KNP), South Africa, using the DNA sequences of the genes coding for major surface protein ( msp1β ) and heat shock protein ( groEL ), respectively. A total of 747 blood samples were collected from February 2014 to August 2016 from African buffaloes kept in KNP, and DNAs were tested using a molecular-phylogenetic approach. Out of 747 samples tested, 129 (17.3%) and 98 (13.1%) were positive for single infection with A. marginale and A. centrale , respectively; whereas 113 (15.1%) were positive for both Anaplasma spp. Pairwise difference of 1.6–8.5% was observed in msp1β sequences of A. marginale whereas that was only 0.3–2.4% for groEL sequences of A. centrale . Separate phylogenetic analyses of msp1β and groEL sequences of A. marginale and A. centrale , respectively, revealed that sequences of Anaplasma spp. from African buffaloes were unique and they grouped separately when compared with previously published sequences of both species. This is the first study to characterise A. marginale and A. centrale from African buffalo using species specific molecular markers. This study will pave the way for future studies to assess genetic variation among Anaplasma spp. from wild ruminants using molecular markers that are better at differentiating between species and strains than the more commonly used 16S rRNA gene, and help to undertake health and fitness studies and host-parasite dynamics using quantitative molecular tools.


  • 주제어

    African buffaloes .   Anaplasma .   msp1β .   groEL .   South Africa.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기