본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

European journal of lipid science and technology : EJLST v.119 no.2, 2017년, pp.1500532 -    SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Application and comparison of high‐speed countercurrent chromatography and high‐performance liquid chromatography in semi‐preparative separation of decarboxymethyl oleuropein aglycone (3,4‐DHPEA‐EDA), a bioactive secoiridoid from extra‐virgin olive oil

Jiménez‐Sánchez, Cecilia (Department of Analytical Chemistry, University of Granada, Granada, Spain ) ; Lozano‐Sánchez, Jesús (Department of Analytical Chemistry, University of Granada, Granada, Spain ) ; Brüggemann, Margret (Institute of Food Chemistry, Technische Universität Braunschweig, Braunschweig, Germany ) ; Neves‐Vieira, Mariana (Institute of Food Chemistry, Technische Universität Braunschweig, Braunschweig, Germany ) ; Rodriguez‐Werner, Miriam (Institute of Food Chemistry, Technische Universität Braunschweig, Braunschweig, Germany ) ; Schmalfuß, Eva (Institute of Food Chemistry, Technische Universität Braunschweig, Braunschweig, Germany ) ; Winterhalter, Peter (Institute of Food Chemistry, Technische Universität Braunschweig, Braunschweig, Germany ) ; Segura‐Carretero, Antonio (Department of Analytical Chemistry, University of Granada, Granada, Spain ) ; Fernández‐Gutiérrez, Alberto (Department of Analytical Chemistry, University of Granada, Granada, Spain ) ;
  • 초록  

    This paper primarily extends research on applying and comparing high‐speed countercurrent chromatography (HSCCC) and high‐performance liquid chromatography (HPLC) in semi‐preparative isolation of decarboxymethyl oleuropein aglycone (3,4‐DHPEA‐EDA), a bioactive secoiridoid, from extra‐virgin olive oil (EVOO). An EVOO phenolic extract (EVOO‐PE) was obtained by solid‐phase extraction (SPE), and this extract was subjected to one run using the maximum loading capacity of each methodology without loss of resolution. For this sample set, the HSCCC method proved to be a more efficient approach regarding loading capacity, solvent consumption, and throughput compared with the HPLC method. However, the purity of the target from HPLC was superior to that of HSCCC, as the number of theoretical plates could not match that of HPLC. Therefore, we propose that HPLC should be used when higher purities are required, despite higher costs. In addition, HPLC is orthogonal and complementary to HSCCC, and can often provide straightforward resolution of otherwise difficult separations by HSCCC. Practical applications : Our results indicate that the optimized HSCCC is an economic process, and hence, a good candidate for further scaling‐up to preparative and industrial scales, as long as the chromatographic profile of the sample itself is simple enough to achieve good resolution, or a very high purity of the compound of interest is not required. For those cases, HPLC would provide superior target purity but would result in higher costs. This paper compares high‐speed countercurrent chromatography (HSCCC) and high‐performance liquid chromatography (HPLC) in semi‐preparative isolation of decarboxymethyl oleuropein aglycone (3,4‐DHPEA‐EDA), a bioactive secoiridoid, from extra‐virgin olive oil (EVOO). An EVOO phenolic extract (EVOO‐PE) was obtained by solid‐phase extraction (SPE), and this extract was subjected to one run using the maximum loading capacity of each methodology without loss of resolution. For this sample set, the HSCCC method proved to be a more efficient approach regarding loading capacity, solvent consumption, and throughput compared with the HPLC method. However, the purity of the target from HPLC was superior to that of HSCCC, as the number of theoretical plates could not match that of HPLC.


  • 주제어

    Extra‐virgin olive oil .   Isolation .   Olea europaea .   Purification .   Separation.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기