Novel anti‐adipogenic properties of the individual trans8,cis10 conjugated linoleic acid (CLA) isomer in 3T3‐L1 adipocytes
In this study, we hypothesized that trans ( t )8, cis ( c )10 conjugated linoleic acid (CLA) isomer possesses anti‐adipogenic properties in resemblance to its structural analog, the t 10, c 12 CLA isomer. After chemical synthesis and purification of the t 8, c 10 CLA isomer in our laboratory, we report, for the first time, its individual action on the lipid metabolism of 3T3‐L1 adipocytes. Cells were supplemented with 50 μM of linoleic acid (LA), t 10, c 12, or t 8, c 10 during 11 days of differentiation. Intracellular triacylglycerols (TAG) content was affected by fatty acid treatments. The t 8, c 10 CLA isomer reduced TAG content but in a lower magnitude, three times less, than the t 10, c 12 isomer. The supplementation with LA increased TAG storage thus reversing CLA isomers effect. The fatty acid profile of treated adipocytes showed that the t 8, c 10 CLA isomer incorporation was identical to the t 10, c 12. The mRNA expression of several genes involved in lipogenesis, lipolysis, fatty acid β‐oxidation, and adipocytes differentiation underlying anti‐adipogenic action was determined. The t 10, c 12 CLA isomer decreased gene expression levels related to lipogenesis but did not up‐regulate HSL and PPARA associated with lipolysis and fatty acid β‐oxidation, respectively. The t 8, c 10 CLA isomer increased SCD mRNA level without affecting delta 9 desaturase index‐16, an indicator of SCD enzyme activity. Taken together, our results indicate that the t 8, c 10 CLA isomer, tested individually on lipid metabolism of 3T3‐L1 adipocytes, is anti‐adipogenic. Practical applications: New and more effective weight loss supplements are widely pursued facing the worldwide obesity epidemic. Over the last few decades, the anti‐adipogenic properties of conjugated linoleic acid (CLA) have been studied and confirmed for the t 10, c 12 CLA isomer but, until now, there was no information on its structural analog, the t 8, c 10. This study provides novel insights on the putative anti‐adipogenic ability of the t 8, c 10 CLA isomer exploiting potential molecular mechanisms of action in 3T3‐L1 adipocytes. The present study reports, for the first time, the anti‐adipogenic properties of the individual t 8, c 10 CLA isomer in 3T3‐L1 adipocytes, obtained through chemical synthesis and purification in our laboratory. mRNA expression levels of several genes involved in potential molecular mechanisms of anti‐adipogenic action, as lipogenesis, lipolysis, fatty acid β‐oxidation and differentiation, were evaluated.
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