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Journal of clinical pathology v.70 no.3, 2017년, pp.260 - 265   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

The usefulness of a novel fully automated PCR-based Idylla test for detection of the BRAF V600E mutation in thyroid tissue: comparison with PNA-clamping PCR, real-time PCR and pyrosequencing

Yeo, Min-Kyung (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea ) ; Jung, Min-Kyu (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea ) ; Lee, Su-Yel (Biobank of Chungnam National University Hospital, , Daejeon, Korea ) ; Lee, Yong-Moon (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea ) ; Hur, Gang Min (Department of Pharmacology, Chungnam National University School of Medicine, , Daejeon, Korea ) ; Kim, Jin-Man (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea ) ;
  • 초록  

    Introduction The BRAF V600E mutation is the most common genetic event in papillary thyroid carcinoma (PTC). The BRAF V600E mutational status has a significant diagnostic and prognostic role in PTC since it can be detected in 32%-87% of PTC by various molecular methods. Aim(s) A novel, fully automated real-time PCR-based Idylla test is assessed to detect the BRAF mutation in formalin-fixed paraffin-embedded (FFPE) thyroid samples. Methods 99 PTC and 11 nodular hyperplasia FFPE thyroid tissues are evaluated for the BRAF V600E mutation by the Idylla tests and compared with peptide nucleic acid-clamping PCR, real-time PCR and pyrosequencing. Results The sensitivity and specificity of the Idylla test to detect BRAF V600E are 98.8% and 100%, which is superior to real-time PCR and pyrosequencing. The concordance between Idylla and true positive is highest at 0.974. Conclusions This study validates that the Idylla test is a sensitive and specific method to detect BRAF V600E in FFPE thyroid tissues. A simple, quick and easy to handle Idylla test is a useful and reliable molecular technique to evaluate BRAF mutations.


  • 주제어

    THYROID .   MOLECULAR PATHOLOGY .   ENDOCRINE PATHOLOGY.  

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