본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Scientific reports v.6, 2016년, pp.37905 -    SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Four translation initiation pathways employed by the leaderless mRNA in eukaryotes

Akulich, Kseniya A. (Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia ) ; Andreev, Dmitry E. (School of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119234, Russia ) ; Terenin, Ilya M. (Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russia ) ; Smirnova, Victoria V. (Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia ) ; Anisimova, Aleksandra S. (Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russia ) ; Makeeva, Desislava S. (School of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119234, Russia ) ; Arkhipova, Valentina I. (Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russia ) ; Stolboushkina, Elena A. (School of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119234, Russia ) ; Garber, Maria B. (Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russia ) ; Prokofjeva, Maria M. (School of Bioengineering and Bioinformatics, Lomono ) ; Spirin, Pavel V. ; Prassolov, Vladimir S. ; Shatsky, Ivan N. ; Dmitriev, Sergey E. ;
  • 초록  

    mRNAs lacking 5′ untranslated regions (leaderless mRNAs) are molecular relics of an ancient translation initiation pathway. Nevertheless, they still represent a significant portion of transcriptome in some taxons, including a number of eukaryotic species. In bacteria and archaea, the leaderless mRNAs can bind non-dissociated 70 S ribosomes and initiate translation without protein initiation factors involved. Here we use the Fleeting mRNA Transfection technique (FLERT) to show that translation of a leaderless reporter mRNA is resistant to conditions when eIF2 and eIF4F, two key eukaryotic translation initiation factors, are inactivated in mammalian cells. We report an unconventional translation initiation pathway utilized by the leaderless mRNA in vitro, in addition to the previously described 80S-, eIF2-, or eIF2D-mediated modes. This mechanism is a bacterial-like eIF5B/IF2-assisted initiation that has only been reported for hepatitis C virus-like internal ribosome entry sites (IRESs). Therefore, the leaderless mRNA is able to take any of four different translation initiation pathways in eukaryotes.


 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기