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Scientific reports v.6, 2016년, pp.37427 -    SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

ECM proteins in a microporous scaffold influence hepatocyte morphology, function, and gene expression

Wang, Yan (School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore ) ; Kim, Myung Hee (Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, 637553, Singapore ) ; Shirahama, Hitomi (School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore ) ; Lee, Jae Ho (Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, 637553, Singapore ) ; Ng, Soon Seng (School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore ) ; Glenn, Jeffrey S. (Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, 637553, Singapore ) ; Cho, Nam-Joon (School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore ) ;
  • 초록  

    It is well known that a three-dimensional (3D) culture environment and the presence of extracellular matrix (ECM) proteins facilitate hepatocyte viability and maintenance of the liver-specific phenotype in vitro. However, it is not clear whether specific ECM components such as collagen or fibronectin differentially regulate such processes, especially in 3D scaffolds. In this study, a series of ECM-functionalized inverted colloidal crystal (ICC) microporous scaffolds were fabricated and their influence on Huh-7.5 cell proliferation, morphology, hepatic-specific functions, and patterns of gene expression were compared. Both collagen and fibronectin promoted albumin production and liver-specific gene expression of Huh-7.5 cells, compared with the bare ICC scaffold. Interestingly, cells in the fibronectin-functionalized scaffold exhibited different aggregation patterns to those in the collagen-functionalized scaffold, a variation that could be related to the distinct mRNA expression levels of cell adhesion-related genes. Based on these results, we can conclude that different ECM proteins, such as fibronectin and collagen, indeed play distinct roles in the phenotypic regulation of cells cultured in a 3D environment.


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