Asthmatic farm children show increased CD3+ CD8low T-cells compared to non-asthmatic farm children
Highlights Asthmatic farm children exhibit more CD3 + CD8 low T-cells compared to non-asthmatic farm children. IFN-γ secretion is decreased in asthmatic compared to non-asthmatic farm-exposed children Asthmatic farm children with asthma risk alleles have more CD3 + CD8 low T-cells than non-asthmatic children with risk alleles. The amount of CD8 low T-cells indicates a specific farm-dependent mechanism associated with childhood asthma.
Abstract Several studies report an important role of CD8 + cytotoxic T-cells in atopy. Farm children show protection against atopy development, partly explained by CD4 + T-cell subtypes. Additional effects of CD8 + T-cells are unknown being investigated in this study within the PASTURE/EFRAIM birth cohort in PBMCs from farming and non-farming 6-year-old ( N = 76) German children. CD3 + CD8 + CD25 + T-cells were analyzed by flow cytometry. Genotyping of 17q21 locus-SNPs associated with childhood asthma was performed. No differences in CD8 + T-cell subsets were seen between farmers and non-farmers regardless of asthma. Among farm children, asthmatics displayed increased CD3 + CD8 low (CD25 + ) T-cells compared to non-asthmatics. Asthmatic farm children exhibited a lower PI-induced stimulatory capacity of CD3 + CD8 low (CD25 + ) cells and a lower IFN-γ secretion than non-asthmatic farm children. Among farm children with GSDMB and ORMDL3 risk alleles, asthmatics displayed higher CD3 + CD8 low cells than non-asthmatics. Our data indicates a specific role of CD8 low T-cells in asthmatic farm children.
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