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Analytical biochemistry v.538, 2017년, pp.1 - 4   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Method to minimize ozone effect on Cy5 fluorescent intensity in DNA microarrays

Kim, Youngjun (Laboratory of Research and Development for Genomics, Cheil General Hospital, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ); Seo, Hyun Hee (Laboratory of Research and Development for Genomics, Cheil General Hospital, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ); Jeong, Mi Seon (Laboratory of Research and Development for Genomics, Cheil General Hospital, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ); Lee, Ki Heon (Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ); Lee, In Ho (Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ); So, Kyeong A. (Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ); Kim, Mi Kyung (Department of Obstetrics and ); Lee, Yoo-Kyung ( ); Kim, Seon Ah ( ); Kim, Tae Jin ( );
  • 초록  

    Abstract Cyanine 5 (Cy5) is an established fluorescent dye in microarray analysis. It is degraded rapidly when exposed to atmospheric ozone during post-hybridization washes, which leads to loss of fluorescent intensity. To minimize this undesirable effect, we coated microarray slides with sodium dodecyl sulfate (SDS) solution at post-hybridization washes. The fluorescent intensities on coated slides were more stable than those on uncoated slide. We also performed the microarrays with SDS solution for a year to check the solution's effectiveness along with seasonal changes of atmospheric ozone level. Consistent results in microarray analysis were obtained using Cy5 dye under atmospheric ozone.


  • 주제어

    DNA microarray .   Cy5 .   Ozone .   SDS coating .   Fluorescent intensity.  

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