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Fungal genetics and biology : FG & B v.108, 2017년, pp.36 - 43   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

The calcium-binding protein EpANN from the lichenized fungus Endocarpon pusillum enhances stress tolerance in yeast and plants

Zhang, Yongli (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10010, PR China ) ; Li, Hui (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10010, PR China ) ; Wang, Yanyan (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10010, PR China ) ; Wei, Jiangchun (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10010, PR China ) ;
  • 초록  

    Abstract Annexins are calcium-phospholipid binding proteins that play a significant role in the Ca 2+ signaling pathway. These proteins are essential for plants to effectively respond to abiotic stresses. However, their functions and mechanisms remain largely unknown in fungi. In this study, an annexin gene, Epann , was cloned from the lichenized fungus Endocarpon pusillum , a drought resistant organism. Our results showed that Epann was induced by several abiotic stresses in E. pusillum . Heterologous expression of the Epann gene enhanced the stress tolerance of Saccharomyces cerevisiae . Under heat-shock conditions, the EpANN proteins were significantly aggregated and the aggregation sites were located on peroxisomes. In heat-shocked cells, Epann reduced the reactive oxygen species level mainly through its intracellular peroxidase activity and regulation of stress-related genes. Transgenic Arabidopsis plants overexpressing Epann exhibited a higher germination rate under oxidative stress and stronger drought tolerance. Our results provide a mechanistic understanding of the role of annexins in abiotic stress responses and suggest that this lichenized fungal gene could be a promising resource to generate stress-tolerant transgenic organisms. Highlights Epann -transgenic yeast had improved abiotic stress resistance, especially to heat. Under heat stress, EpANN-GFP protein accumulated in peroxisomes in transgenic yeast. Epann reduced ROS levels mainly through intracellular peroxidase activity. Transgenic Arabidopsis germinated better under stress and had drought tolerance.


  • 주제어

    Lichenized fungus .   Abiotic stress tolerance .   Heat shock .   Gene overexpression .   Heterologous expression.  

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