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Experimental cell research v.361 no.1, 2017년, pp.135 - 140   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Fecal bacteria from treatment-naive Crohn's disease patients can skew helper T cell responses

Ma, Fei (Department of Oncology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China ) ; Zhang, Yi (Department of Digestive Endoscopic Diagnosis and Treatment, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China ) ; Xing, Junjie (Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China ) ; Song, Xiaoling (Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China ) ; Huang, Ling (Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China ) ; Weng, Hao (Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China ) ; Wu, Xiangsong (Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China ) ; Walker, Emma (DICAT Biomedical Computation Centre, Vancouver, Canada ) ; Wang, Zhongchuan (Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, Ch ) ;
  • 초록  

    Abstract Many studies have demonstrated that the inflamed mucosa of Crohn's disease (CD) patients presented a disturbed gut commensal community, and the shift in microbial composition and species variety is associated with disease severity. To establish a link between changes in the intestinal bacterial composition and the alteration of inflammation, we obtained fecal bacteria from CD patients and non-CD controls. The bacteria were then used to stimulate the peripheral blood mononuclear cells (PBMCs) from one non-CD individual. We found that the frequency of IFN-γ- and IL-17-expressing CD4 T cells was significantly higher after stimulation with CD bacteria than with non-CD bacteria, while the frequency of IL-4- and IL-10-expressing CD4 T cells was significantly decreased after stimulation with CD bacteria. A similar trend was observed in the level of cytokine expression and transcription expression. However, this difference was not clear-cut, as overlapping regions were observed between the two groups. With longer stimulation using CD bacteria, the skewing toward Th1/Th17 responses were further increased. This increase depended on the presence of monocytes/macrophages. Interestingly, we also found that B cells presented an inhibitory effect in CD bacteria-mediated skewing toward Th1/Th17 cells and promoted IL-10 secretion in CD bacteria-stimulated PBMCs. Together, our results demonstrated that CD bacteria could promote Th1/Th17 inflammation in a host factor-independent fashion.


  • 주제어

    Bacteria .   Crohn's disease .   Helper T cell.  

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