Carvacrol promotes angiogenic paracrine potential and endothelial differentiation of human mesenchymal stem cells at low concentrations
Abstract Objectives Phenolic monoterpene compound, named Carvacrol, has been found to exert different biological outcomes. It has been accepted that the angiogenic activity of human mesenchymal stem cells was crucial in the pursuit of appropriate regeneration. In the current experiment, we investigated the contribution of Carvacrol on the angiogenic behavior of primary human mesenchymal stem cells. Methods Mesenchymal stem cells were exposed to Carvacrol in a dose ranging from 25 to 200μM for 48h. We measured cell survival rate by MTT assay and migration rate by a scratch test. The oxidative status was monitored by measuring SOD, GPx activity. The endothelial differentiation was studied by evaluating the level of VE-cadherin and vWF by real-time PCR and ELISA analyses. The content of VEGF and tubulogenesis behavior was monitored in vitro . We also conducted Matrigel plug in vivo CAM assay to assess the angiogenic potential of conditioned media from human mesenchymal stem cells after exposure to Carvacrol. Results Carvacrol was able to increase mesenchymal stem cell survival and migration rate ( p p in vitro ( p Conclusions Our data shed lights on the possibility of a Carvacrol to induce angiogenesis in human mesenchymal stem cells by modulating cell differentiation and paracrine angiogenic response. Highlights Carvacrol promotes human mesenchymal stem cells viability, antioxidant activity and migration in vitro . Carvacrol stimulates trans‑differentiation of stem cells into endothelial lineage by inducing vWF and VE-cadherin. Angiocrine activity of stem cells is improved in the presence of Carvacrol by the secretion of VEGF. Condition media prepared from Carvacrol-exposed cells induce angiogenesis in vivo .
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