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Biochemical and biophysical research communications v.494 no.3/4, 2017년, pp.575 - 580   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Structure insights into the molecular mechanism of the interaction between UHRF2 and PCNA

Chen, Wanbiao (Hefei National Laboratory for Physical Sciences at Microscale CAS Center for Excellence in Biomacromolecules, Collaborative Innovation Center of Chemistry for Life Sciences, School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China ) ; Wu, Minhao (Hefei National Laboratory for Physical Sciences at Microscale CAS Center for Excellence in Biomacromolecules, Collaborative Innovation Center of Chemistry for Life Sciences, School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China ) ; Hang, Tianrong (Hefei National Laboratory for Physical Sciences at Microscale CAS Center for Excellence in Biomacromolecules, Collaborative Innovation Center of Chemistry for Life Sciences, School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China ) ; Wang, Chengliang (Hefei National Laboratory for Physical Sciences at Microscale CAS Center for Excellence in Biomacromolecules, Collaborative ) ; Zhang, Xuan ; Zang, Jianye ;
  • 초록  

    Abstract UHRF2 (Ubiquitin-like with PHD and ring finger domains 2) is an E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications and cell cycle regulation by interacting with multiple epigenetic or cell-cycle related proteins. Previous studied have identified PCNA (Proliferating cell nuclear antigen) as an interacting partner of UHRF2 by using the antibody microarray. However, the molecular mechanism and the function of UHRF2-PCNA interaction remains unclear. Here, we report the complex structure of PCNA and the peptide ( 784 NEIL QTLLDLFF PGYSK 800 ) derived from UHRF2 that contains a PIP box. Structural analysis combined with mutagenesis experiments provide the molecular basis for the recognition of UHRF2 by PCNA via PIP-box. Highlights UHRF2 interacts with PCNA through its PIP box. Complex structure of PCNA/UHRF2 PIP reveals the molecular mechanism of how PCNA recognizes UHRF2. UHRF2-PIP box interacts with PCNA mainly via hydrophobic interaction. The hydrogen bond formed between PCNA-H44 and UHRF2 PIP -D792 is critical for the interaction.


  • 주제어

    UHRF2 .   PIP-box .   PCNA .   Structure .   Molecular mechanism.  

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